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Sample GSM7474562 Query DataSets for GSM7474562
Status Public on May 01, 2024
Title DT 24h
Sample type RNA
Source name Diphtheria-Toxin treated+ 24h recovery
Organism Mus musculus
Characteristics genotype: Atp4b-DTR
tissue: Gastric Corpus Tissue
Treatment protocol Diphtheria toxin (225ng/mouse) or saline vehicle was administered via ip injuection daily for three days. Mice were euthanized at 24, 36, 48, or 72 hours after final siphtheria toxin injection
Growth protocol Mice were grown in a temperature and light controlled mouse facility; littermate controls were used when possible. Adult mice age 8-12 weeks were used.
Extracted molecule total RNA
Extraction protocol Total RNA extracted, purified by RNEasy Micro Kit, and RNA intergrity verified on Agilent Bioanalyzer
Label Biotin
Label protocol Total RNA was extracted and used to prepare biotinylated cDNA with Affymetrix WT plus reagent kit
Hybridization protocol Labelled cDNA hybridized to Affymetrix GeneChip Mouse Gene 2.0 ST Arrays according to manufacturers instructions and placed in GeneChip Hybridization Oven 620 for 18hr at 45C. Microarrays were washes and stained with Affymettrix Fluidics Station 450.
Scan protocol Arrays were scanned with a GeneChip® 7G Scanner 3000 laser confocal slide scanner
Data processing Microarray data data expression was process using Command Console (Affymetrix, Inc) and the raw .CEL files were analyzed using Partek Genomic Suite v7.0 (Partek Inc., St Louis, MO). Microarray CEL files were imported into Partek and normalized using RMA.
Submission date Jun 12, 2023
Last update date May 01, 2024
Contact name Jason C Mills
Phone 713-798-4882
Organization name Baylor College of Medicine
Street address 1 Baylor Plaza
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platform ID GPL16570
Series (1)
GSE234777 Microarray analysis of regenerating parietal cells (PCs) in murine gastric epithelium recovering from Diphtheria toxin injury

Data table header descriptions
VALUE Quantification

Data table
17200001 6.21432
17200003 5.58942
17200005 2.75144
17200007 4.75731
17200009 4.97645
17200011 3.31958
17200013 4.40536
17200015 5.60443
17200017 2.6743
17200019 2.94514
17200021 3.41575
17200023 5.40333
17200025 4.80553
17200027 5.07544
17200029 4.45197
17200031 4.00748
17200033 3.17298
17200035 3.61587
17200037 5.41862
17200039 2.63936

Total number of rows: 41345

Table truncated, full table size 682 Kbytes.

Supplementary file Size Download File type/resource
GSM7474562_DT_24h.CEL.gz 8.3 Mb (ftp)(http) CEL
GSM7474562_DT_24h.chp.gz 251.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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