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| Status |
Public on Mar 11, 2024 |
| Title |
acuteCtrl299(MTA-1_0) |
| Sample type |
RNA |
| |
|
| Source name |
C57/Bl6
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
Sex: Male
treatment: Ctrl
tissue: liver
|
| Treatment protocol |
The mice were stressed with our Cvs protocol as previously described (doi:https://doi.org/10.1016/j.molmet.2018.06.012). In brief, the Cvs protocoll consists of five different stressors (warm swim, cold exposure, shaking, restraint and overnight isolation) applied in a random manner 2 per day for 15 days. the first cohort was analysed immediately after the stress protocoll, a recovery cohor was analysed 3 month later. Age matched unstressed animals were used as control pe cohort.
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| Growth protocol |
C57/Bl6 male mice were bred and maintained in a regular 12h light/dark cycle (22°C ± 1°C, 50% ± 5% humidity). All mice were group housed (max. 5 mice per cage) with ad libitum access to standard chow (58% carbohydrates, 9% fat, 33% protein, R/M-H Extrudat, ssniff Spezialdiäten GmbH, Soest, Germany) and tap water. Animal protocols were approved by the Landesamt für Natur, Umwelt und Verbraucherschutz (LANUV), NRW, Germany (81-02.04.2017.A421) and performed according to the ‘Principle of laboratory animal care’ (NIH publication No. 85-23, revised 1996) and the German law on the protection of animals.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction from liver tissue was made with RNeasy mini kit (Qiagen, Hilden, Germany) including DNAse digestion according to the manufacturer's instructions. RNA quality was evaluated using an Agilent 2100 Bioanalyzer and only high quality RNA (RIN > 8) was used for microarray analysis.
|
| Label |
biotin
|
| Label protocol |
150 ng total RNAwas amplified using the WT Expression Kit and the WT Terminal Labeling Kit (Thermofisher, Darmstadt, Germany).
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| |
|
| Hybridization protocol |
Amplified cDNA was hybridized on Affymetrix Mouse MTA arrays (Affymetrix)
|
| Scan protocol |
Detection of probe intensities was performed using a GeneChip scanner 3000 7G (GDAS 1.4 package, Affymetrix).
|
| Description |
RNA expression value derived from Expression Console software
|
| Data processing |
The data were analyzed with Affymetrix Expression Console v4.0 using default analysis settings for combined RMA analyses dabg .CHP files.
|
| |
|
| Submission date |
Jun 14, 2023 |
| Last update date |
Mar 11, 2024 |
| Contact name |
Birgit Knebel |
| Organization name |
German Diabetes Center
|
| Department |
Cinical Biochemistry and Pathobiochemistry
|
| Street address |
Auf'm Hennekamp 65
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21877 |
| Series (2) |
| GSE234944 |
Chronic stress alters hepatic metabolism and thermodynamic respiratory efficiency affecting epigenetics in C57BL/6 mice [MTA array, Affymetrix] |
| GSE235424 |
Chronic stress alters hepatic metabolism and thermodynamic respiratory efficiency affecting epigenetics in C57BL/6 mice |
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