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Sample GSM7487958 Query DataSets for GSM7487958
Status Public on Jun 14, 2024
Title single L7 motor neurons, AGE 1, Control, replicate 3
Sample type SRA
 
Source name single L7 motor neurons
Organism Aplysia californica
Characteristics tissue: single L7 motor neurons
age: 8 months
genotype: Wild Type
treatment: Control
Treatment protocol Short-term sensitization, long-term sensitization, untrained controls
Growth protocol Three different age groups (8,10, 12 months old)of Aplysia california maintained at National Aplysia Resource Facility, University of Miami.
Extracted molecule total RNA
Extraction protocol Total RNAs from single L7 motor neuron (L7MN) were isolated for RNAseq after one hour of behavior training.
Clontech SMART-Seq Ultra Low Input RNA kit, following manufacturer's protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description neuron_1A8
Data processing Reads were assessed for quality, trimmed if PHRED scores fell below 20, and any remaining adapter sequences were removed
Processed paired-end reads were aligned to human reference genome GRCh38 using HISAT2, and counts were generated using the HTseq analysis package
Reads that successfully mapped to the reference genome were used for downstream analyses
Assembly: GCF_000002075.1_AplCal3
Supplementary files format and content: htseq output with gene counts for each sample
 
Submission date Jun 14, 2023
Last update date Jun 14, 2024
Contact name Carrie McCracken
E-mail(s) cmccracken@som.umaryland.edu
Organization name Institute of Genome
Department Institute for Genome Sciences
Street address 670 W. Baltimore St
City Baltimore
State/province MD
ZIP/Postal code 21201
Country USA
 
Platform ID GPL21596
Series (1)
GSE234983 Single neuron analysis of aging associated changes in learning reveals escalating impairments in transcriptional plasticity
Relations
BioSample SAMN35736716
SRA SRX20680818

Supplementary file Size Download File type/resource
GSM7487958_neuron_1A8.sorted_by_name.exon.counts.txt.gz 74.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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