|
| Status |
Public on Jun 14, 2024 |
| Title |
single L7 motor neurons, AGE 1, Control, replicate 6 |
| Sample type |
SRA |
| |
|
| Source name |
single L7 motor neurons
|
| Organism |
Aplysia californica |
| Characteristics |
tissue: single L7 motor neurons
age: 8 months
genotype: Wild Type
treatment: Control
|
| Treatment protocol |
Short-term sensitization, long-term sensitization, untrained controls
|
| Growth protocol |
Three different age groups (8,10, 12 months old)of Aplysia california maintained at National Aplysia Resource Facility, University of Miami.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs from single L7 motor neuron (L7MN) were isolated for RNAseq after one hour of behavior training.
Clontech SMART-Seq Ultra Low Input RNA kit, following manufacturer's protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
neuron_2A29
|
| Data processing |
Reads were assessed for quality, trimmed if PHRED scores fell below 20, and any remaining adapter sequences were removed
Processed paired-end reads were aligned to human reference genome GRCh38 using HISAT2, and counts were generated using the HTseq analysis package
Reads that successfully mapped to the reference genome were used for downstream analyses
Assembly: GCF_000002075.1_AplCal3
Supplementary files format and content: htseq output with gene counts for each sample
|
| |
|
| Submission date |
Jun 14, 2023 |
| Last update date |
Jun 14, 2024 |
| Contact name |
Carrie McCracken |
| E-mail(s) |
cmccracken@som.umaryland.edu
|
| Organization name |
Institute of Genome
|
| Department |
Institute for Genome Sciences
|
| Street address |
670 W. Baltimore St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL21596 |
| Series (1) |
| GSE234983 |
Single neuron analysis of aging associated changes in learning reveals escalating impairments in transcriptional plasticity |
|
| Relations |
| BioSample |
SAMN35736713 |
| SRA |
SRX20680821 |
