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Sample GSM750 Query DataSets for GSM750
Status Public on Nov 28, 2001
Title SAGE_SciencePark_MCF7_Control_0h
Sample type SAGE
Anchor NlaIII
Tag Count 61593
Tag Length 10
Source name Breast Carcinoma - Pleaural effusion
Organism Homo sapiens
Extracted molecule total RNA
Description Estrogen receptor positive MCF7 cells were grown in conditions devoid of estrogen for 48h (i.e. IMEM media supplemented with 10% Charcoal Stripped Serum). This SAGE library represents the 0h time point (i.e. baseline, untreated cells) of an estrogen exposure time course experiment.
Sex: Female
Organ: Breast
Cell_type: epithelial
Cell_line: MCF7
Lab host: Top10 bacteria
Vector: pZero
Vector type: plasmid
R. Site 1: NlaIII
R. Site 2: BsmF1 as tagging enzyme
Sequences generated to date: 3055

This library represents a Cancer Genome Anatomy Project library, which was either produced through CGAP funding, or donated to CGAP.
The Cancer Genome Anatomy Project (CGAP: is an interdisciplinary program established and administered by the National Cancer Institute (NCI: to generate the information and technological tools needed to decipher the molecular anatomy of the cancer cell.
Keywords = mammary gland
Keywords = carcinoma
Keywords = cell line
Keywords = EST
Submission date Nov 28, 2001
Last update date May 28, 2005
Contact name C Marcelo Aldaz
Phone 512-237-9530
Organization name M.D. Anderson Cancer Center
Department Science Park Research Division, Carcinogenesis
Street address
City Smithville
State/province TX
ZIP/Postal code 78957
Country USA
Platform ID GPL4
Series (1)
GSE41 Effects of estrogen

Data table header descriptions
TAG Ten base SAGE tag,
COUNT Absolute tag count
TPM Tags per million, or (1000000*COUNT)/(Total tags)

Data table
CCTCCAGCTA 776 12598.8
CCAGAACAGA 582 9449.13
CTGGGTTAAT 526 8539.93
TTGGTCCTCT 524 8507.46
ATAATTCTTT 523 8491.22
GCATAATAGG 513 8328.87
TACCATCAAT 445 7224.85
ACTTTTTCAA 414 6721.54
CACAAACGGT 397 6445.54
GGGAAGCAGA 375 6088.35
GTGCTGAATG 373 6055.88
CAAACCATCC 369 5990.94
AAGACAGTGG 357 5796.11
GGAAAAAAAA 354 5747.41
AAGGTGGAGG 347 5633.76
GTGAAGGCAG 343 5568.81
AGGCTACGGA 337 5471.4
ACATCATCGA 307 4984.33
AGCACCTCCA 303 4919.39
CAAATGAGGA 289 4692.09

Total number of rows: 15965

Table truncated, full table size 328 Kbytes.

Supplementary data files not provided

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