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Sample GSM750258 Query DataSets for GSM750258
Status Public on Aug 25, 2011
Title E18th rat embryonic striatal stem cells (EGF+FGF+IGF)_1
Sample type RNA
Source name Cultured Embryonic progenitors from Striatum (group D)
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
developmental stage: E18
cell type: embryonic striatal stem cell
treatment: EGF+FGF+IGF
Treatment protocol Growth factor supplements were replaced as of 50% in every second day and passaging was performed every 4/5th day. In culture, ENP-derived neurospheres were passaged every 5th days into a single cell suspension (P = passage).
Growth protocol ESSCs were cultured in 25 cm2 flasks at a density of 1 X 105 to 3 X 105/ ml in neural basal medium (Stem cell technologies) with the previously discussed growth factor conditions and incubated at 370c, 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA samples were isolated from triplicates of three groups (treatment A, D & E), using Qiagen miRNeasy Mini Kit.
Label Biotin
Label protocol 1000 ng of total RNA was Poly (A) tailed and ligated to a biotinylated signal molecule using flash tag biotin HSA RNA Labeling Kit.
Hybridization protocol The biotinylated target RNA sample was then hybridized to Affymetrix miRNA arrays for 16 hours at 480C with rotation at 60 rpm according to GeneChip Hybridization. The GeneChip® miRNA Array probeset was used for miRNA profiling.
Scan protocol The scanned images were inspected for hybridization efficiency and, CEL files generated from AGCC (Affymetrix GeneChip Command Console) were imported into miRNA quality control QC Tool software for determination of data quality.
Description miRNA expression data from Group D-derived ESSCs
Group D ESSCs
Data processing miRNA array data analysis was performed with the Partek Genomics SuiteTM software.
For evaluation of miRNA expression data, Affymetrix CEL files were imported into the software prior to analysis.
Expression values calculated for rno-miRNAs.
Submission date Jun 28, 2011
Last update date Aug 25, 2011
Contact name SOUMYA PATI
Street address KUBANG KERIAN
ZIP/Postal code 16150
Country Malaysia
Platform ID GPL8786
Series (1)
GSE30276 miRNA expression profiles from E18th rat embryonic striatal stem cells under conditioned environments

Data table header descriptions
VALUE RMA signal intensity

Data table
rno-let-7a_st 13.7099
rno-let-7b-star_st 5.94773
rno-let-7b_st 14.3799
rno-let-7c_st 14.8744
rno-let-7d-star_st 6.80428
rno-let-7d_st 14.133
rno-let-7e-star_st 4.25577
rno-let-7e_st 13.1132
rno-let-7f_st 10.5462
rno-let-7i-star_st 4.86782
rno-let-7i_st 13.6183
rno-miR-1-star_st 2.15743
rno-miR-100_st 10.814
rno-miR-101a-star_st 2.82562
rno-miR-101a_st 2.26401
rno-miR-101b_st 5.334
rno-miR-103_st 13.3888
rno-miR-106b-star_st 9.3018
rno-miR-106b_st 12.8123
rno-miR-107_st 13.1647

Total number of rows: 351

Table truncated, full table size 8 Kbytes.

Supplementary file Size Download File type/resource
GSM750258.CEL.gz 177.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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