|
| Status |
Public on Jun 30, 2023 |
| Title |
AGS Cells, BYL719 Treated, Biol Rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
AGS
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: AGS
cell type: gastric adenocarcinoma
genotype: WT
treatment: 1 uM BYL719, 24 hours
|
| Treatment protocol |
Cells were treated for 24 hours with 1 uM BYL719 (Selleckchem)
|
| Growth protocol |
AGS cells were maintained in DMEM/F-12K nutrient medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini kit (Qiagen)
RNA library was prepared by Novogene (Beijing, China) following their protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Read assembly, trimming and QC (performed by third party)
HISAT2
DESeq2
Assembly: hg38
Supplementary files format and content: aligned reads as bam file for each sample
Supplementary files format and content: DESeq analysis files
|
| |
|
| Submission date |
Jun 22, 2023 |
| Last update date |
Jun 30, 2023 |
| Contact name |
Micah Luftig |
| E-mail(s) |
micah.luftig@duke.edu
|
| Organization name |
Duke University
|
| Street address |
213 Research Dr, 0045 CARL
|
| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE235631 |
CBF-beta mitigates PI3K-alpha specific inhibitor killing through PIM1 in PIK3CA mutant gastric cancer |
|
| Relations |
| BioSample |
SAMN35845449 |
| SRA |
SRX20753887 |
