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Sample GSM7506607 Query DataSets for GSM7506607
Status Public on Jun 30, 2023
Title AGS cells, untreated, BYL719-Resistant Cl.3
Sample type SRA
 
Source name AGS
Organism Homo sapiens
Characteristics cell line: AGS
cell type: gastric adenocarcinoma
genotype: WT
treatment: None
Treatment protocol Cells were treated for 24 hours with 1 uM BYL719 (Selleckchem)
Growth protocol AGS cells were maintained in DMEM/F-12K nutrient medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) in humidified atmosphere with 5% CO2 at 37C
Extracted molecule total RNA
Extraction protocol RNA was harvested using Rneasy mini kit (Qiagen)
RNA library was prepared by Novogene (Beijing, China) following their protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Read assembly, trimming and QC (performed by third party)
HISAT2
DESeq2
Assembly: hg38
Supplementary files format and content: aligned reads as bam file for each sample
Supplementary files format and content: DESeq analysis files
 
Submission date Jun 22, 2023
Last update date Jun 30, 2023
Contact name Micah Luftig
E-mail(s) micah.luftig@duke.edu
Organization name Duke University
Street address 213 Research Dr, 0045 CARL
City Durham
State/province North Carolina
ZIP/Postal code 27710
Country USA
 
Platform ID GPL24676
Series (1)
GSE235631 CBF-beta mitigates PI3K-alpha specific inhibitor killing through PIM1 in PIK3CA mutant gastric cancer
Relations
BioSample SAMN35845445
SRA SRX20753891

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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