|
Status |
Public on Jun 30, 2023 |
Title |
AGS cells, untreated, BYL719-Resistant Cl.3 |
Sample type |
SRA |
|
|
Source name |
AGS
|
Organism |
Homo sapiens |
Characteristics |
cell line: AGS cell type: gastric adenocarcinoma genotype: WT treatment: None
|
Treatment protocol |
Cells were treated for 24 hours with 1 uM BYL719 (Selleckchem)
|
Growth protocol |
AGS cells were maintained in DMEM/F-12K nutrient medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) in humidified atmosphere with 5% CO2 at 37C
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Rneasy mini kit (Qiagen) RNA library was prepared by Novogene (Beijing, China) following their protocol
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Read assembly, trimming and QC (performed by third party) HISAT2 DESeq2 Assembly: hg38 Supplementary files format and content: aligned reads as bam file for each sample Supplementary files format and content: DESeq analysis files
|
|
|
Submission date |
Jun 22, 2023 |
Last update date |
Jun 30, 2023 |
Contact name |
Micah Luftig |
E-mail(s) |
micah.luftig@duke.edu
|
Organization name |
Duke University
|
Street address |
213 Research Dr, 0045 CARL
|
City |
Durham |
State/province |
North Carolina |
ZIP/Postal code |
27710 |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (1) |
GSE235631 |
CBF-beta mitigates PI3K-alpha specific inhibitor killing through PIM1 in PIK3CA mutant gastric cancer |
|
Relations |
BioSample |
SAMN35845445 |
SRA |
SRX20753891 |