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Sample GSM7508729 Query DataSets for GSM7508729
Status Public on Apr 19, 2024
Title ASPS_Tumor-5
Sample type SRA
 
Source name ASPS tumor
Organism Homo sapiens
Characteristics tissue: ASPS tumor
Treatment protocol In indicated conditions, cells and xenografts were treated with palobciclib for 24 hours or 3 days, respectively.
Growth protocol Dulbecco’s modified Eagle’s medium containing 10% FBS, 2 mM L- glutamine, 100 mg/ml penicillin, and 100 mg/ml streptomycin
Extracted molecule polyA RNA
Extraction protocol Total RNA was isolated using an RNeasy Plus Kit (Qiagen).
Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs Cat# E7645S). Paired-end 150 bp sequencing was performed on a NovaSeq 6000 (Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing fastq files wre aligned using STAR, and transcript quantitation performed using cufflinks.
Assembly: hg19
Supplementary files format and content: Gene expresion in FPKM are in text format
 
Submission date Jun 26, 2023
Last update date Apr 19, 2024
Contact name Matthew Hemming
E-mail(s) matthew.hemming@umassmemorial.org
Organization name UMass Medical School
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platform ID GPL24676
Series (2)
GSE235738 Alveolar Soft Part Sarcoma is driven by ASPSCR1::TFE3-dependent and therapeutically targetable transcriptional programs [RNA-Seq]
GSE235739 ASPSCR1::TFE3 Drives Alveolar Soft Part Sarcoma by Inducing Targetable Transcriptional Programs
Relations
BioSample SAMN35984489
SRA SRX20777907

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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