|
| Status |
Public on Jul 05, 2023 |
| Title |
F4/80+HGF_1day_biologycal rep_2 |
| Sample type |
RNA |
| |
|
| Source name |
Kupffer cells RNA
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Cultivation of Kupffer cells for 1 day with MCSF and with HGF for 1 day.
|
| Treatment protocol |
Macrophages samples were cellected on different time periods after cocultivation with Ito cells, CD146cells, HGF/cultivation with MCSF
|
| Growth protocol |
Macrophages samples were collected, frozen, lysed in QIAZOL reagent for the following RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ordinary extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7g
|
| Description |
Gene expression data from cultivation of Kupffer cells for 1 day with MCSF and with HGF for 1 day
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) Software version 4.0.2 using Affymetrix default analysis settings and RMA algorithm
|
| |
|
| Submission date |
Jul 03, 2023 |
| Last update date |
Jul 05, 2023 |
| Contact name |
Polina Vishnyakova |
| E-mail(s) |
vpa2002@mail.ru
|
| Organization name |
National Medical Research Center for Obstetrics, Gynecology and Perinatology
|
| Street address |
Oparina 4
|
| City |
Moscow |
| ZIP/Postal code |
116571 |
| Country |
Russia |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE236447 |
Gene expression profile of macrophages of monocytic origin and Kupffer cells under conditions of co-cultivation with Ito cells, endotheliocytes of sinusoidal capillaries of the liver and HGF. |
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