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Sample GSM7546715 Query DataSets for GSM7546715
Status Public on Jul 05, 2023
Title CD115 + Ito cells_1 day_biologycal rep_1
Sample type RNA
 
Source name monocytic macrophages RNA
Organism Mus musculus
Characteristics treatment: Cultivation of monocytic macrophages for 1 day with MCSF and with Ito cells for 1 day.
Treatment protocol Macrophages samples were cellected on different time periods after cocultivation with Ito cells, CD146cells, HGF/cultivation with MCSF
Growth protocol Macrophages samples were collected, frozen, lysed in QIAZOL reagent for the following RNA extraction
Extracted molecule total RNA
Extraction protocol Ordinary extraction of total RNA was performed according to the manufacturer's instructions (Qiagen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7g
Description Gene expression data from cultivation of monocytic macrophages for 1 day with MCSF and with Ito cells for 1 day
Data processing The data were analyzed with Transcriptome Analysis Console (TAC) Software version 4.0.2 using Affymetrix default analysis settings and RMA algorithm
 
Submission date Jul 03, 2023
Last update date Jul 05, 2023
Contact name Polina Vishnyakova
E-mail(s) vpa2002@mail.ru
Organization name National Medical Research Center for Obstetrics, Gynecology and Perinatology
Street address Oparina 4
City Moscow
ZIP/Postal code 116571
Country Russia
 
Platform ID GPL23038
Series (1)
GSE236447 Gene expression profile of macrophages of monocytic origin and Kupffer cells under conditions of co-cultivation with Ito cells, endotheliocytes of sinusoidal capillaries of the liver and HGF.

Data table header descriptions
ID_REF
VALUE Quantification
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
AFFX-BkGr-GC03_st 5.13974 0.814216
AFFX-BkGr-GC04_st 5.11203 0.873915
AFFX-BkGr-GC05_st 5.34216 0.891915
AFFX-BkGr-GC06_st 5.06471 0.917206
AFFX-BkGr-GC07_st 4.38152 0.925042
AFFX-BkGr-GC08_st 3.63083 0.923544
AFFX-BkGr-GC09_st 3.34277 0.909932
AFFX-BkGr-GC10_st 3.15727 0.891584
AFFX-BkGr-GC11_st 2.83716 0.872403
AFFX-BkGr-GC12_st 2.66416 0.849277
AFFX-BkGr-GC13_st 2.32531 0.888065
AFFX-BkGr-GC14_st 2.17207 0.87775
AFFX-BkGr-GC15_st 2.02407 0.877788
AFFX-BkGr-GC16_st 2.1355 0.846437
AFFX-BkGr-GC17_st 2.20374 0.826783
AFFX-BkGr-GC18_st 2.63702 0.75351
AFFX-BkGr-GC19_st 4.59987 0.629385
AFFX-BkGr-GC20_st 4.83796 0.625279
AFFX-BkGr-GC21_st 4.93915 0.615544
AFFX-BkGr-GC22_st 4.95375 0.611943

Total number of rows: 28846

Table truncated, full table size 1048 Kbytes.




Supplementary file Size Download File type/resource
GSM7546715_13B_Clariom_S_Mouse_.CEL.gz 1.0 Mb (ftp)(http) CEL
GSM7546715_13B_Clariom_S_Mouse_.sst-rma-gene-full.chp.gz 320.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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