|
| Status |
Public on Apr 27, 2012 |
| Title |
testis_ko_3 |
| Sample type |
RNA |
| |
|
| Source name |
16 dpp testis, knockout
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Tex19.1-/-
gender: male
tissue: testis
developmental stage: 16 days post partum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen), and then treated with DNase I. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Knockout animal 3.
Biological replicate 3 of 4.
|
| Data processing |
Background subtracted using Illumina BeadStudio, then log-transformed, quantile-normalized using lumi in R.
|
| |
|
| Submission date |
Jul 06, 2011 |
| Last update date |
Apr 27, 2012 |
| Contact name |
Ian Adams |
| Organization name |
MRC Human Genetics Unit
|
| Department |
Genome Regulation
|
| Street address |
Crewe Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH42XU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE30461 |
Gene expression in 16 dpp Tex19.1-/- mouse testes |
|
