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Sample GSM755847 Query DataSets for GSM755847
Status Public on May 11, 2012
Title HeLa CTR RNAi replicate 2 A
Sample type RNA
 
Source name Luciferase knockdown
Organism Homo sapiens
Characteristics cell line: HeLa cells
batch: A
RNAi: Luciferase
Treatment protocol siRNAs were transfected using Oligofectamine and OPTIMEM (Invitrogen) according to suppliers protocol. 2x10^5 cells at 40-50% confluency were transfected with 200 pmol siRNA and collected 72h later.
Growth protocol HeLa cells were cultured at 37°C in Dulbecco´s Modified Eagle´s Medium (Invitrogen) supplemented with 10% fetal calf serum (Invitrogen) and penicillin/streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from HeLa cells employing the RNeasy Kit (Qiagen) with additional RNase-free DNase (Qiagen) on column digestion. The quality and quantity of the RNA was determined using the Agilent RNA 6000 NanoLabChip kit and Agilent 2100 bioanalyzer.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description HeLa cells; Luciferase knockdown; biological replicate 2
Data processing R/bioconductor, 'oligo' package, default parameters
 
Submission date Jul 07, 2011
Last update date May 11, 2012
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address Großhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL6244
Series (1)
GSE30471 The nucleosome remodeler SNF2L modulates WNT signaling for cellular homeostasis

Data table header descriptions
ID_REF
VALUE RMA signals calculated using 'oligo' package of bioconductor

Data table
ID_REF VALUE
7892501 4.457
7892502 5.540
7892503 3.357
7892504 8.624
7892505 2.106
7892506 3.715
7892507 4.704
7892508 6.685
7892509 11.750
7892510 2.907
7892511 2.010
7892512 6.620
7892513 3.394
7892514 9.140
7892515 7.465
7892516 5.801
7892517 7.363
7892518 2.160
7892519 5.246
7892520 8.377

Total number of rows: 33297

Table truncated, full table size 456 Kbytes.




Supplementary file Size Download File type/resource
GSM755847.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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