|
| Status |
Public on May 11, 2012 |
| Title |
HeLa CTR RNAi replicate 3 A |
| Sample type |
RNA |
| |
|
| Source name |
Luciferase knockdown
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa cells
batch: A
RNAi: Luciferase
|
| Treatment protocol |
siRNAs were transfected using Oligofectamine and OPTIMEM (Invitrogen) according to suppliers protocol. 2x10^5 cells at 40-50% confluency were transfected with 200 pmol siRNA and collected 72h later.
|
| Growth protocol |
HeLa cells were cultured at 37°C in Dulbecco´s Modified Eagle´s Medium (Invitrogen) supplemented with 10% fetal calf serum (Invitrogen) and penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from HeLa cells employing the RNeasy Kit (Qiagen) with additional RNase-free DNase (Qiagen) on column digestion. The quality and quantity of the RNA was determined using the Agilent RNA 6000 NanoLabChip kit and Agilent 2100 bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
HeLa cells; Luciferase knockdown; biological replicate 3
|
| Data processing |
R/bioconductor, 'oligo' package, default parameters
|
| |
|
| Submission date |
Jul 07, 2011 |
| Last update date |
May 11, 2012 |
| Contact name |
Tobias Straub |
| E-mail(s) |
tstraub@med.uni-muenchen.de
|
| Organization name |
LMU Munich
|
| Department |
Biomedical Center, Bioinformatics
|
| Street address |
Großhadener Str. 9
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE30471 |
The nucleosome remodeler SNF2L modulates WNT signaling for cellular homeostasis |
|
