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Sample GSM755848 Query DataSets for GSM755848
Status Public on May 11, 2012
Title HeLa CTR RNAi replicate 3 A
Sample type RNA
Source name Luciferase knockdown
Organism Homo sapiens
Characteristics cell line: HeLa cells
batch: A
RNAi: Luciferase
Treatment protocol siRNAs were transfected using Oligofectamine and OPTIMEM (Invitrogen) according to suppliers protocol. 2x10^5 cells at 40-50% confluency were transfected with 200 pmol siRNA and collected 72h later.
Growth protocol HeLa cells were cultured at 37°C in Dulbecco´s Modified Eagle´s Medium (Invitrogen) supplemented with 10% fetal calf serum (Invitrogen) and penicillin/streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from HeLa cells employing the RNeasy Kit (Qiagen) with additional RNase-free DNase (Qiagen) on column digestion. The quality and quantity of the RNA was determined using the Agilent RNA 6000 NanoLabChip kit and Agilent 2100 bioanalyzer.
Label biotin
Label protocol standard Affymetrix protocol
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description HeLa cells; Luciferase knockdown; biological replicate 3
Data processing R/bioconductor, 'oligo' package, default parameters
Submission date Jul 07, 2011
Last update date May 11, 2012
Contact name Tobias Straub
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address Großhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
Platform ID GPL6244
Series (1)
GSE30471 The nucleosome remodeler SNF2L modulates WNT signaling for cellular homeostasis

Data table header descriptions
VALUE RMA signals calculated using 'oligo' package of bioconductor

Data table
7892501 5.686
7892502 5.649
7892503 5.026
7892504 8.698
7892505 2.862
7892506 4.323
7892507 4.786
7892508 6.723
7892509 11.785
7892510 3.142
7892511 2.626
7892512 6.360
7892513 3.054
7892514 10.307
7892515 7.531
7892516 4.836
7892517 7.817
7892518 4.264
7892519 5.694
7892520 8.626

Total number of rows: 33297

Table truncated, full table size 457 Kbytes.

Supplementary file Size Download File type/resource
GSM755848.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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