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Sample GSM755850 Query DataSets for GSM755850
Status Public on May 11, 2012
Title HeLa SNF2L RNAi replicate 2
Sample type RNA
 
Source name SNF2L knockdown
Organism Homo sapiens
Characteristics cell line: HeLa cells
batch: B
RNAi: SNF2L
Treatment protocol siRNAs were transfected using Oligofectamine and OPTIMEM (Invitrogen) according to suppliers protocol. 2x10^5 cells at 40-50% confluency were transfected with 200 pmol siRNA and collected 72h later.
Growth protocol HeLa cells were cultured at 37°C in Dulbecco´s Modified Eagle´s Medium (Invitrogen) supplemented with 10% fetal calf serum (Invitrogen) and penicillin/streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from HeLa cells employing the RNeasy Kit (Qiagen) with additional RNase-free DNase (Qiagen) on column digestion. The quality and quantity of the RNA was determined using the Agilent RNA 6000 NanoLabChip kit and Agilent 2100 bioanalyzer.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description HeLa cells; SNF2L knockdown; biological replicate 2
Data processing R/bioconductor, 'oligo' package, default parameters
 
Submission date Jul 07, 2011
Last update date May 11, 2012
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address Großhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL6244
Series (1)
GSE30471 The nucleosome remodeler SNF2L modulates WNT signaling for cellular homeostasis

Data table header descriptions
ID_REF
VALUE RMA signals calculated using 'oligo' package of bioconductor

Data table
ID_REF VALUE
7892501 7.175
7892502 5.045
7892503 2.218
7892504 8.763
7892505 2.452
7892506 4.193
7892507 3.769
7892508 4.076
7892509 11.202
7892510 2.735
7892511 2.346
7892512 8.224
7892513 3.383
7892514 10.852
7892515 7.824
7892516 5.409
7892517 5.155
7892518 2.322
7892519 6.048
7892520 8.419

Total number of rows: 33297

Table truncated, full table size 456 Kbytes.




Supplementary file Size Download File type/resource
GSM755850.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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