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Sample GSM755854 Query DataSets for GSM755854
Status Public on May 11, 2012
Title HeLa CTR RNAi replicate 3 B
Sample type RNA
 
Source name Luciferase knockdown
Organism Homo sapiens
Characteristics cell line: HeLa cells
batch: B
RNAi: Luciferase
Treatment protocol siRNAs were transfected using Oligofectamine and OPTIMEM (Invitrogen) according to suppliers protocol. 2x10^5 cells at 40-50% confluency were transfected with 200 pmol siRNA and collected 72h later.
Growth protocol HeLa cells were cultured at 37°C in Dulbecco´s Modified Eagle´s Medium (Invitrogen) supplemented with 10% fetal calf serum (Invitrogen) and penicillin/streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from HeLa cells employing the RNeasy Kit (Qiagen) with additional RNase-free DNase (Qiagen) on column digestion. The quality and quantity of the RNA was determined using the Agilent RNA 6000 NanoLabChip kit and Agilent 2100 bioanalyzer.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description HeLa cells; Luciferase knockdown; biological replicate 3
Data processing R/bioconductor, 'oligo' package, default parameters
 
Submission date Jul 07, 2011
Last update date May 11, 2012
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address Großhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL6244
Series (1)
GSE30471 The nucleosome remodeler SNF2L modulates WNT signaling for cellular homeostasis

Data table header descriptions
ID_REF
VALUE RMA signals calculated using 'oligo' package of bioconductor

Data table
ID_REF VALUE
7892501 6.123
7892502 4.584
7892503 2.604
7892504 8.635
7892505 1.866
7892506 4.140
7892507 3.051
7892508 3.310
7892509 11.211
7892510 2.537
7892511 2.360
7892512 7.661
7892513 1.914
7892514 10.473
7892515 7.600
7892516 4.673
7892517 4.748
7892518 2.571
7892519 5.583
7892520 8.387

Total number of rows: 33297

Table truncated, full table size 456 Kbytes.




Supplementary file Size Download File type/resource
GSM755854.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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