|
| Status |
Public on Nov 05, 2023 |
| Title |
293T,3days, rep2_3 |
| Sample type |
SRA |
| |
|
| Source name |
HEK293T
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
cell type: Human embryonic kidney
genotype: Wild type
treatment: Nucleofection
|
| Treatment protocol |
4.5 μg all-in-one plasmid for each target site, expressing Cas-SF01 and designed crRNA, and 1 nM of annealed GUIDE-seq oligonucleotides were transfected into HEK293T cells (2×106) by electroporation (Lonza, V4XC-2012). The electroporation was performed by 4D-Nucleofector™ Core Unit with program CM-130, and each sample was repeated three times and then cultured into one plate. Cells were then collected at approximately 90% confluency after transfection and stored at -80°C after liquid nitrogen flash freezing.
|
| Growth protocol |
DMEM supplemented with 10% (v/v) fetal bovine serum and 1% Penicillin‒Streptomycin at 37°C with 5% CO2.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
the sequencing data were collected and analyzed by DIATRE, Shanghai.
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Cas-SF01_rep3_3
|
| Data processing |
*library strategy: GUIDE-seq
GUIDE-seq was analysed using an open-source guideseq software:
https://github.com/aryeelab/guideseq
Assembly: hg38
|
| |
|
| Submission date |
Jul 07, 2023 |
| Last update date |
Nov 05, 2023 |
| Contact name |
Weilong Yang |
| E-mail(s) |
yangweilong@whu.edu.cn
|
| Organization name |
Southern University of Science and Technology
|
| Street address |
No. 1088 Xueyuan Avenue, Nanshan District
|
| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518055 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE236755 |
Cas-SF01, an engineered Cas12i nuclease is an efficient genome editing tool in mammalian cells and plants |
|
| Relations |
| BioSample |
SAMN36347271 |
| SRA |
SRX20929989 |
