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Sample GSM7575051 Query DataSets for GSM7575051
Status Public on Nov 05, 2023
Title 293T,3days, rep2_4
Sample type SRA
 
Source name HEK293T
Organism Homo sapiens
Characteristics cell line: HEK293T
cell type: Human embryonic kidney
genotype: Wild type
treatment: Nucleofection
Treatment protocol 4.5 μg all-in-one plasmid for each target site, expressing Cas-SF01 and designed crRNA, and 1 nM of annealed GUIDE-seq oligonucleotides were transfected into HEK293T cells (2×106) by electroporation (Lonza, V4XC-2012). The electroporation was performed by 4D-Nucleofector™ Core Unit with program CM-130, and each sample was repeated three times and then cultured into one plate. Cells were then collected at approximately 90% confluency after transfection and stored at -80°C after liquid nitrogen flash freezing.
Growth protocol DMEM supplemented with 10% (v/v) fetal bovine serum and 1% Penicillin‒Streptomycin at 37°C with 5% CO2.
Extracted molecule genomic DNA
Extraction protocol the sequencing data were collected and analyzed by DIATRE, Shanghai.
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina NovaSeq 6000
 
Description Cas-SF01_rep4_4
Data processing *library strategy: GUIDE-seq
GUIDE-seq was analysed using an open-source guideseq software:
https://github.com/aryeelab/guideseq
Assembly: hg38
 
Submission date Jul 07, 2023
Last update date Nov 05, 2023
Contact name Weilong Yang
E-mail(s) yangweilong@whu.edu.cn
Organization name Southern University of Science and Technology
Street address No. 1088 Xueyuan Avenue, Nanshan District
City Shenzhen
State/province Guangdong
ZIP/Postal code 518055
Country China
 
Platform ID GPL24676
Series (1)
GSE236755 Cas-SF01, an engineered Cas12i nuclease is an efficient genome editing tool in mammalian cells and plants
Relations
BioSample SAMN36347270
SRA SRX20929990

Supplementary file Size Download File type/resource
GSM7575051_rep2_TTR_identifiedOfftargets.txt.gz 17.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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