|
Status |
Public on Sep 06, 2023 |
Title |
Male 1 SARS2 [X21260] |
Sample type |
SRA |
|
|
Source name |
brain
|
Organism |
Mus musculus |
Characteristics |
tissue: brain genotype: Wild-type C57BL/6 Sex: male age: 10 month treatment: SARS2
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from tissue homogenate using Trizol™ LS reagent (Thermo Fisher scientific) according to the manufacturer’s instructions. Library preparation was performed using a TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Raw sequencing reads were evaluated and trimmed using Trimmomatic Trimmed reads were mapped to the mouse reference genome GRCm38 using HISAT2 RNA-seq raw counts for each gene were extracted using FeatureCounts Assembly: GRCm38 Supplementary files format and content: xlsx file includes raw counts for each sample
|
|
|
Submission date |
Jul 11, 2023 |
Last update date |
Sep 06, 2023 |
Contact name |
Ling Li |
E-mail(s) |
lil@umn.edu
|
Organization name |
University of Minnesota
|
Department |
Experimental and Clinical Pharmacology
|
Lab |
Li Lab
|
Street address |
Mcguire Translational Research Facility, 4-419
|
City |
Minneapolis |
State/province |
MN |
ZIP/Postal code |
55455 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE237092 |
Impact of Age and Sex on neuroinflammation due to SARS-CoV2 infection in a murine model. |
|
Relations |
BioSample |
SAMN36408215 |
SRA |
SRX20984743 |