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Sample GSM7594669 Query DataSets for GSM7594669
Status Public on Sep 06, 2023
Title Female 1 SARS2 [X21252]
Sample type SRA
 
Source name brain
Organism Mus musculus
Characteristics tissue: brain
genotype: Wild-type C57BL/6
Sex: female
age: 10 month
treatment: SARS2
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from tissue homogenate using Trizol™ LS reagent (Thermo Fisher scientific) according to the manufacturer’s instructions.
Library preparation was performed using a TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Raw sequencing reads were evaluated and trimmed using Trimmomatic
Trimmed reads were mapped to the mouse reference genome GRCm38 using HISAT2
RNA-seq raw counts for each gene were extracted using FeatureCounts
Assembly: GRCm38
Supplementary files format and content: xlsx file includes raw counts for each sample
 
Submission date Jul 11, 2023
Last update date Sep 06, 2023
Contact name Ling Li
E-mail(s) lil@umn.edu
Organization name University of Minnesota
Department Experimental and Clinical Pharmacology
Lab Li Lab
Street address Mcguire Translational Research Facility, 4-419
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platform ID GPL24247
Series (1)
GSE237092 Impact of Age and Sex on neuroinflammation due to SARS-CoV2 infection in a murine model.
Relations
BioSample SAMN36408212
SRA SRX20984734

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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