|
| Status |
Public on Sep 06, 2023 |
| Title |
Female 2 SARS2 [X21195] |
| Sample type |
SRA |
| |
|
| Source name |
brain
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
genotype: Wild-type C57BL/6
Sex: female
age: 10 month
treatment: SARS2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissue homogenate using Trizol™ LS reagent (Thermo Fisher scientific) according to the manufacturer’s instructions.
Library preparation was performed using a TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer’s instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw sequencing reads were evaluated and trimmed using Trimmomatic
Trimmed reads were mapped to the mouse reference genome GRCm38 using HISAT2
RNA-seq raw counts for each gene were extracted using FeatureCounts
Assembly: GRCm38
Supplementary files format and content: xlsx file includes raw counts for each sample
|
| |
|
| Submission date |
Jul 11, 2023 |
| Last update date |
Sep 06, 2023 |
| Contact name |
Ling Li |
| E-mail(s) |
lil@umn.edu
|
| Organization name |
University of Minnesota
|
| Department |
Experimental and Clinical Pharmacology
|
| Lab |
Li Lab
|
| Street address |
Mcguire Translational Research Facility, 4-419
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE237092 |
Impact of Age and Sex on neuroinflammation due to SARS-CoV2 infection in a murine model. |
|
| Relations |
| BioSample |
SAMN36408211 |
| SRA |
SRX20984735 |
