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Sample GSM759497 Query DataSets for GSM759497
Status Public on Jul 14, 2011
Title [E-MTAB-513] HCT20161, breast, female, 29 years, 2x50 paired end mRNA-seq
Sample type SRA
Source name breast, female, 29 years, 2x50 paired end mRNA-seq
Organism Homo sapiens
Characteristics organismpart: breast
Sex: female
age: 29 years
phenotype: caucasian
material type: total_RNA
libraryprep: mRNA-Seq
biosourceprovider: Human breast total RNA, lot 04060013
Extracted molecule total RNA
Extraction protocol nucleic_acid_extraction | 1ug of total RNA was subjected to two rounds of oligo-dT beads binding. Purified mRNA was fragmented and random primed for cDNA synthesis. cDNA fragements were end repared, added dATP, and liagted to illumina pair end sample prep adaptor. The ligated material was amplified by PCR for 15 cycles, and used for illumina sequencing.
sequencing | Illumina sequencing was performed at HiSeq 2000 at Illumina
bioassay_data_transformation | FASTQ files (Illumina format) of sequencing reads were retrieved from the sequencing machine
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description 50bp_PE_mRNA_Seq_FCB_s_4_1_sequence
2x50 paired end mRNA-seq READ1
~ 0.5% phiX DNA spiked in
Performer: ILLUMINA-CA
Data processing processed data not provided
Submission date Jul 12, 2011
Last update date May 15, 2019
Organization European Bioinformatics Institute
Lab ArrayExpress
Street address Wellcome Trust Genome Campus
City Hinxton
State/province Cambridgeshire
ZIP/Postal code CB10 1SD
Country United Kingdom
Platform ID GPL11154
Series (2)
GSE30611 [E-MTAB-513] Illumina Human Body Map 2.0 Project
GSE49417 The conserved organization of the human and mouse transcriptomes
Reanalyzed by GSE97211
SRA ERX011225

Supplementary data files not provided
SRA Run SelectorHelp
Processed data not provided for this record
Raw data are available in SRA

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