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Sample GSM759647 Query DataSets for GSM759647
Status Public on Sep 14, 2011
Title DHT siFoxA1 rep1_v3
Sample type RNA
 
Source name DHT siFoxA1
Organism Homo sapiens
Characteristics cell line: LNCaP-1F5
genotype/variation: FoxA1 depleted
Treatment protocol FoxA1 depletion was done using siRNA smartpool specific to FoxA1 mRNA. Both parental and FoxA1 depleted cells were grown in charcoal stripped medium for 3 days followed by treated with 100 nM 5a-dihydrotestosterone (DHT) or 100 nM DEX for 24 hours followed by RNA isolation.
Growth protocol LNCaP-1F5 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 10 mM HEPES supplemented with antibiotics (penicillin and streptomycin).
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kitwith DNaseI treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scan protocol
Description 5050496020_G, HumanHT-12_V3
Data processing The data were normalised using quantile normalisation with BeadStudio Program from Illumina.
 
Submission date Jul 12, 2011
Last update date Sep 14, 2011
Contact name Olli A. Jänne
E-mail(s) olli.janne@helsinki.fi
Phone +358919125040
Organization name University of Helsinki
Department Inst. of Biomedicine/Physiology
Lab Androgen Receptor Laboratory
Street address Haartmaninkatu-8
City Helsinki
ZIP/Postal code FI-00014
Country Finland
 
Platform ID GPL6947
Series (2)
GSE30622 Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer [Expression Array]
GSE30624 Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 8 0.21739
ILMN_2055271 23 0.05929
ILMN_1736007 7.4 0.23452
ILMN_2383229 -10.7 0.82082
ILMN_1806310 8.8 0.20158
ILMN_1779670 -9.2 0.78393
ILMN_2321282 -2.1 0.51779
ILMN_1671474 -5.1 0.63109
ILMN_1772582 -4.7 0.6087
ILMN_1735698 -3.7 0.57181
ILMN_1653355 14.5 0.11067
ILMN_1717783 -5 0.62319
ILMN_1705025 -9.1 0.77602
ILMN_1814316 -18.3 0.96047
ILMN_2359168 6.6 0.24506
ILMN_1731507 -23.3 0.99209
ILMN_1787689 16.8 0.09223
ILMN_1745607 -13.5 0.88274
ILMN_2136495 -3.9 0.57576
ILMN_1668111 -0.4 0.43742

Total number of rows: 48794

Table truncated, full table size 1182 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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