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Sample GSM759654 Query DataSets for GSM759654
Status Public on Sep 14, 2011
Title Vehicle siFoxA1 rep2_v4
Sample type RNA
 
Source name Vehicle siFoxA1
Organism Homo sapiens
Characteristics cell line: LNCaP-1F5
genotype/variation: FoxA1 depleted
Treatment protocol FoxA1 depletion was done using siRNA smartpool specific to FoxA1 mRNA. Both parental and FoxA1 depleted cells were grown in charcoal stripped medium for 3 days followed by treated with 100 nM 5a-dihydrotestosterone (DHT) or 100 nM DEX for 24 hours followed by RNA isolation.
Growth protocol LNCaP-1F5 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 10 mM HEPES supplemented with antibiotics (penicillin and streptomycin).
Extracted molecule total RNA
Extraction protocol RNA was extracted with QIAGEN RNeasy mini kitwith DNaseI treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scan protocol
Description 5417622013_F, HumanHT-12_V4
Data processing The data were normalised using quantile normalisation with BeadStudio Program from Illumina.
 
Submission date Jul 12, 2011
Last update date Sep 14, 2011
Contact name Olli A. Jänne
E-mail(s) olli.janne@helsinki.fi
Phone +358919125040
Organization name University of Helsinki
Department Inst. of Biomedicine/Physiology
Lab Androgen Receptor Laboratory
Street address Haartmaninkatu-8
City Helsinki
ZIP/Postal code FI-00014
Country Finland
 
Platform ID GPL10558
Series (2)
GSE30622 Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer [Expression Array]
GSE30624 Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 -4.7 0.57922
ILMN_2055271 17.9 0.08961
ILMN_1736007 -8.4 0.72468
ILMN_2383229 -9.4 0.76364
ILMN_1806310 0.4 0.40909
ILMN_1779670 -19.8 0.96623
ILMN_1653355 20.1 0.06234
ILMN_1717783 -23.8 0.99091
ILMN_1705025 11.7 0.16623
ILMN_1814316 11.8 0.16104
ILMN_2359168 -4.7 0.58182
ILMN_1731507 -21.2 0.97273
ILMN_1787689 -11.9 0.82078
ILMN_3241953 13.8 0.13636
ILMN_1745607 -4.9 0.58831
ILMN_2136495 -16.8 0.92468
ILMN_1668111 -25.2 0.9961
ILMN_2295559 2.6 0.35714
ILMN_1735045 -1.6 0.47922
ILMN_1680754 1.9 0.37532

Total number of rows: 47231

Table truncated, full table size 1128 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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