|
Status |
Public on Sep 14, 2011 |
Title |
DEX siFoxA1 rep1_v4 |
Sample type |
RNA |
|
|
Source name |
DEX siFoxA1
|
Organism |
Homo sapiens |
Characteristics |
cell line: LNCaP-1F5 genotype/variation: FoxA1 depleted
|
Treatment protocol |
FoxA1 depletion was done using siRNA smartpool specific to FoxA1 mRNA. Both parental and FoxA1 depleted cells were grown in charcoal stripped medium for 3 days followed by treated with 100 nM 5a-dihydrotestosterone (DHT) or 100 nM DEX for 24 hours followed by RNA isolation.
|
Growth protocol |
LNCaP-1F5 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 10 mM HEPES supplemented with antibiotics (penicillin and streptomycin).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kitwith DNaseI treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scan protocol
|
Description |
5417622013_G, HumanHT-12_V4
|
Data processing |
The data were normalised using quantile normalisation with BeadStudio Program from Illumina.
|
|
|
Submission date |
Jul 12, 2011 |
Last update date |
Sep 14, 2011 |
Contact name |
Olli A. Jänne |
E-mail(s) |
olli.janne@helsinki.fi
|
Phone |
+358919125040
|
Organization name |
University of Helsinki
|
Department |
Inst. of Biomedicine/Physiology
|
Lab |
Androgen Receptor Laboratory
|
Street address |
Haartmaninkatu-8
|
City |
Helsinki |
ZIP/Postal code |
FI-00014 |
Country |
Finland |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE30622 |
Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer [Expression Array] |
GSE30624 |
Dual Role of FoxA1 in Androgen Receptor Binding to Chromatin, Androgen Signaling and Prostate Cancer |
|