|
| Status |
Public on Sep 27, 2012 |
| Title |
anti-sense GH transgene, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
heterozygous transgenic dwarf rats bearing an anti-sense GH transgene fed ad libitum
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: white adipose tissue
genotype: Jcl:Wistar (-/-) rat
age: 24 weeks
|
| Treatment protocol |
All rats were provided food 30 minutes prior to turning off the lights in the evening and were sacrificed after turning on the lights in the following morning.
|
| Growth protocol |
From six weeks of age, (-/-) rats were divided into two groups: ad libitum (AL) and CR (70% of the energy intake). AL, CR and Tg rats were sacrificed at 24 weeks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the epididymal WAT using TRIZOL (Life Technologies).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
HA_050630_1
|
| Data processing |
PM data of CEL file was parametrically normalized, and expression levels at each gene was estimated by trimmed means of the normalized PM data (Konishi (2008), Statistical Applications in Genetics and Molecular Biology: Vol. 7: Iss. 1, Article 25.) The normalized probe-level PM data is available as a supplementary txt file for each Sample, where the columns of data represent the probes 1-12 in same order to that of the chip's CDF file.
A pseudo data was then derived in a form of antilog of the z-scores; the center of the pseudo data was 256. The pseudo data, ABS_CALL, and the normalized data (z-scores) are presented in the matrix form.
|
| |
|
| Submission date |
Jul 14, 2011 |
| Last update date |
Sep 27, 2012 |
| Contact name |
Tomokazu Konishi |
| E-mail(s) |
konishi@akita-pu.ac.jp
|
| Phone |
+81-18-872-1603
|
| Organization name |
Akita Prefectural University
|
| Department |
Bioresource Sciences
|
| Lab |
Molecular Genetics
|
| Street address |
Shimoshinjyo Nishi
|
| City |
Akita |
| State/province |
Akita |
| ZIP/Postal code |
010-0195 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE30668 |
Life-long caloric restriction-associated remodeling of rat white adipose tissue |
|
