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Sample GSM760652 Query DataSets for GSM760652
Status Public on Sep 27, 2012
Title ad libitum, biological rep3
Sample type RNA
Source name wild Wistar rats fed ad libitum
Organism Rattus norvegicus
Characteristics tissue: white adipose tissue
genotype: Jcl:Wistar (-/-) rat
age: 24 weeks
Treatment protocol All rats were provided food 30 minutes prior to turning off the lights in the evening and were sacrificed after turning on the lights in the following morning.
Growth protocol From six weeks of age, (-/-) rats were divided into two groups: ad libitum (AL) and CR (70% of the energy intake). AL, CR and Tg rats were sacrificed at 24 weeks of age.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the epididymal WAT using TRIZOL (Life Technologies).
Label biotin
Label protocol standard Affymetrix protocol
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description WA_050623_3
Data processing PM data of CEL file was parametrically normalized, and expression levels at each gene was estimated by trimmed means of the normalized PM data (Konishi (2008), Statistical Applications in Genetics and Molecular Biology: Vol. 7: Iss. 1, Article 25.) The normalized probe-level PM data is available as a supplementary txt file for each Sample, where the columns of data represent the probes 1-12 in same order to that of the chip's CDF file.
A pseudo data was then derived in a form of antilog of the z-scores; the center of the pseudo data was 256. The pseudo data, ABS_CALL, and the normalized data (z-scores) are presented in the matrix form.
Submission date Jul 14, 2011
Last update date Sep 27, 2012
Contact name Tomokazu Konishi
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
Platform ID GPL1355
Series (1)
GSE30668 Life-long caloric restriction-associated remodeling of rat white adipose tissue

Data table header descriptions
VALUE pseudo data (antilog of the z-scores)

Data table
1367452_at 5008.6 P 1.874
1367453_at 2431.7 P 1.419
1367454_at 2914.5 P 1.533
1367455_at 5285.3 P 1.908
1367456_at 6250.2 P 2.014
1367457_at 2075.6 P 1.319
1367458_at 702.6 P 0.6362
1367459_at 8798.9 P 2.229
1367460_at 7633.8 P 2.14
1367461_at 2038.4 P 1.307
1367462_at 4801 P 1.847
1367463_at 2834.4 P 1.515
1367464_at 1701.6 P 1.194
1367465_at 2514.2 P 1.44
1367466_at 2698 P 1.484
1367467_at 6309.9 P 2.02
1367468_at 1116.9 P 0.9283
1367469_at 11434.7 P 2.394
1367470_at 4513.4 P 1.808
1367471_at 2148.6 P 1.341

Total number of rows: 31099

Table truncated, full table size 792 Kbytes.

Supplementary file Size Download File type/resource
GSM760652.CEL.gz 2.4 Mb (ftp)(http) CEL
GSM760652.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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