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Sample GSM760655 Query DataSets for GSM760655
Status Public on Sep 27, 2012
Title caloric restriction, biological rep2
Sample type RNA
Source name wild Wistar rats, caloric restriction to 70% of the energy intake
Organism Rattus norvegicus
Characteristics tissue: white adipose tissue
genotype: Jcl:Wistar (-/-) rat
age: 24 weeks
Treatment protocol All rats were provided food 30 minutes prior to turning off the lights in the evening and were sacrificed after turning on the lights in the following morning.
Growth protocol From six weeks of age, (-/-) rats were divided into two groups: ad libitum (AL) and CR (70% of the energy intake). AL, CR and Tg rats were sacrificed at 24 weeks of age.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the epididymal WAT using TRIZOL (Life Technologies).
Label biotin
Label protocol standard Affymetrix protocol
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description WC_050625_2
Data processing PM data of CEL file was parametrically normalized, and expression levels at each gene was estimated by trimmed means of the normalized PM data (Konishi (2008), Statistical Applications in Genetics and Molecular Biology: Vol. 7: Iss. 1, Article 25.) The normalized probe-level PM data is available as a supplementary txt file for each Sample, where the columns of data represent the probes 1-12 in same order to that of the chip's CDF file.
A pseudo data was then derived in a form of antilog of the z-scores; the center of the pseudo data was 256. The pseudo data, ABS_CALL, and the normalized data (z-scores) are presented in the matrix form.
Submission date Jul 14, 2011
Last update date Sep 27, 2012
Contact name Tomokazu Konishi
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
Platform ID GPL1355
Series (1)
GSE30668 Life-long caloric restriction-associated remodeling of rat white adipose tissue

Data table header descriptions
VALUE pseudo data (antilog of the z-scores)

Data table
1367452_at 4447.8 P 1.799
1367453_at 2475.7 P 1.43
1367454_at 2963.7 P 1.543
1367455_at 6659.7 P 2.054
1367456_at 6384.7 P 2.027
1367457_at 1914.6 P 1.268
1367458_at 741.5 P 0.6702
1367459_at 9650.5 P 2.287
1367460_at 6606.7 P 2.048
1367461_at 1939.6 P 1.276
1367462_at 4433.8 P 1.797
1367463_at 3250.7 P 1.602
1367464_at 1900 P 1.263
1367465_at 2773.4 P 1.501
1367466_at 2640.5 P 1.471
1367467_at 6298.7 P 2.018
1367468_at 1321.4 P 1.034
1367469_at 10950 P 2.367
1367470_at 4253.3 P 1.771
1367471_at 2088.3 P 1.323

Total number of rows: 31099

Table truncated, full table size 793 Kbytes.

Supplementary file Size Download File type/resource
GSM760655.CEL.gz 2.4 Mb (ftp)(http) CEL
GSM760655.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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