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Sample GSM762012 Query DataSets for GSM762012
Status Public on May 02, 2012
Title Chicken_HH25_BS2.1
Sample type SRA
 
Source name frontonasal prominence neural crest cells
Organism Gallus gallus
Characteristics # embryos pooled: 5
developmental stage: HH25
species: chicken
Growth protocol Eggs were incubated at 37C until specified Hamburger-Hamilton developmental stage. Frontonasal mesenchyme was dissected as previously described (PMID 20015954).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol (Invitrogen) per manufacturer's instructions. Adapters were ligated to mature miRNAs using the Illumina Small RNA Sample Prep Kit per manufacturers instructions (v1.5 sRNA 3’ Adapter). RNA species from 20-40bp were size selected using a 6% Novex TBE Page gel (Invitrogen)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina Genome Analyzer IIx
 
Description biological replicate of Chicken_HH25_BS1; technical replicate of Chicken_HH25_BS2.2
Data processing Adapter sequences were trimmed from reads and total reads per sequence were calculated using a custom Perl script. The resultant data were then uploaded to the miRAnalyzer program, version 1 (http://web.bioinformatics.cicbiogune.es/microRNA/miRanalyser.php). Processed data are presented as reads per million total reads (PMMR).
 
Submission date Jul 15, 2011
Last update date May 15, 2019
Contact name Kara Powder
E-mail(s) kara.powder@gmail.com
Organization name Washington University in St Louis
Department Genetics
Lab Lovett
Street address 4566 Scott Ave
City Saint Louis
State/province MO
ZIP/Postal code 63108
Country USA
 
Platform ID GPL13797
Series (1)
GSE30716 A cross-species analysis of microRNAs in the developing avian face
Relations
SRA SRX097277
BioSample SAMN00717572

Supplementary file Size Download File type/resource
GSM762012_Chicken_HH25_BS2.1_PMMR.txt.gz 3.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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