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Sample GSM7623435 Query DataSets for GSM7623435
Status Public on Dec 06, 2023
Title NC3
Sample type SRA
Source name gastrocnemius muscle
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: gastrocnemius muscle
treatment: none
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TrizolĀ® Reagent (Invitrogen, USA) according to the instruction manual. Purified RNA was quantified at OD260nm using a ND-1000 spectrophotometer (Nanodrop Technology,USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 LabChip kit (Agilent Technology, USA).
All RNA sample preparation procedures were carried out according to the Illumina's official protocol. SureSelect XT HS2 mRNA Library Preparation kit (Agilent, USA) was used for library construction followed by AMPure XP beads (Beckman Coulter, USA) size selection.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing The sequence was determined using Illumina's sequencing-by-synthesis (SBS) technology (Illumina, USA).
Sequencing data (FASTQ reads) were generated using Welgene Biotech's pipeline based on Illumina's basecalling program bcl2fastq v2.20.
Alignment Tool: HISAT2
Submission date Jul 17, 2023
Last update date Dec 06, 2023
Contact name Yu-Jung Cheng
Organization name China Medical University
Department Physical Therapy
Street address Road NaN-Tong, sec 1, 152-8, 8F
City Taichung
State/province OTH
ZIP/Postal code 403
Country Taiwan
Platform ID GPL24247
Series (1)
GSE237537 A Non-invasive Mouse Model That Recapitulates Disuse-Induced Muscle Atrophy in Immobilized Patients
BioSample SAMN36499501
SRA SRX21050853

Supplementary file Size Download File type/resource
GSM7623435_Gene_TPM_NC3.xlsx 2.5 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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