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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 06, 2023 |
| Title |
NC3 |
| Sample type |
SRA |
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| Source name |
gastrocnemius muscle
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| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: gastrocnemius muscle
treatment: none
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol® Reagent (Invitrogen, USA) according to the instruction manual. Purified RNA was quantified at OD260nm using a ND-1000 spectrophotometer (Nanodrop Technology,USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 LabChip kit (Agilent Technology, USA).
All RNA sample preparation procedures were carried out according to the Illumina's official protocol. SureSelect XT HS2 mRNA Library Preparation kit (Agilent, USA) was used for library construction followed by AMPure XP beads (Beckman Coulter, USA) size selection.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
The sequence was determined using Illumina's sequencing-by-synthesis (SBS) technology (Illumina, USA).
Sequencing data (FASTQ reads) were generated using Welgene Biotech's pipeline based on Illumina's basecalling program bcl2fastq v2.20.
Alignment Tool: HISAT2
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| Submission date |
Jul 17, 2023 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Yu-Jung Cheng |
| Organization name |
China Medical University
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| Department |
Physical Therapy
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| Street address |
Road NaN-Tong, sec 1, 152-8, 8F
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| City |
Taichung |
| State/province |
OTH |
| ZIP/Postal code |
403 |
| Country |
Taiwan |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE237537 |
A Non-invasive Mouse Model That Recapitulates Disuse-Induced Muscle Atrophy in Immobilized Patients |
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| Relations |
| BioSample |
SAMN36499501 |
| SRA |
SRX21050853 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7623435_Gene_TPM_NC3.xlsx |
2.5 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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