|Public on Oct 02, 2023
|Day3-DMSO, CD11c+I-AI-Ehigh cells day3_2
|GM-CSF Bone marrow culture
|cell type: Bone marrrow derived dendritic cells
cell subset: CD11c+I-AI-Ehigh
treatment: DMSO for 3 days
library type: NEB Next Ultra II Directional RNA Library Prep Kit
|DMSO or YPPP added to the dish of bone marrow culture at day 0.
|Bone marrow cells from femur of C57BL/6 mice were cultured with 25 ng/ml GM-CSF and DMSO or YPPP.
|Total RNA of the dendritic cells were extracted with AllPrep DNA/RNA kit (QIAGEN) according to manufacturer instructions.
The libraries were constructed using NEB Next Ultra II Directional RNA Library Prep Kit according to manufacturer instructions.
|Illumina NextSeq 500
|Day3-DMSO, CD11c+I-AI-Ehigh cells
|The fastq fieles were generated using bcl2fastq software.
Sequenced reads were mapped to hg38 using HISAT2 (v2.1.0) with default parameter.
Reads Per Kilobase of exon per Megabase of library size (RPKM) and Transcripts per Million (TPM) were calcurated by StringTie (v1.3.4d) with default parameter.
Supplementary files format and content: tab-delimited text files include FPKM and TPM values for each Sample
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample
|Jul 26, 2023
|Last update date
|Oct 02, 2023
|Kanazawa Medical University Hospital
|Center for Clinical Genomics
|Transcriptome analysis of high potent DCs using small molecule compounds