|
Status |
Public on Mar 20, 2024 |
Title |
GS123_Placenta |
Sample type |
SRA |
|
|
Source name |
Placenta, placebo exposed, 24hrs post intra-amniotic injection
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague-Dawley tissue: placenta gestational day: Day 18 treatment: PBS time: 24 hrs post-injection
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Total RNA was extracted from tissue homogenates with QIAGEN Rneasy kit per manufacturer's intructions After polyA selection, paired-end RNA-sequencing with 150bp was done with a depth of 30M reads.Library was generated at BGI Genomics.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
DNBSEQ-G400 |
|
|
Description |
GS123_Placenta
|
Data processing |
Alignment with RN6.0 genome build with Kallisto Gene summarization and feature counts with tximport Differental expression with DESeq2 Assembly: Rn6.0
|
|
|
Submission date |
Jul 26, 2023 |
Last update date |
Mar 20, 2024 |
Contact name |
Augusto F Schmidt |
E-mail(s) |
aschmidt@med.miami.edu
|
Phone |
3052430156
|
Organization name |
University of Miami Miller School of Medicine
|
Street address |
1611 NW 12th ave
|
City |
Miami |
State/province |
FL |
ZIP/Postal code |
33136 |
Country |
USA |
|
|
Platform ID |
GPL30103 |
Series (1) |
GSE239349 |
Transcriptional responses of the fetal lung after intra-amniotic LPS injection in rats [placenta] |
|
Relations |
BioSample |
SAMN36715876 |
SRA |
SRX21164288 |