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Sample GSM7663518 Query DataSets for GSM7663518
Status Public on Mar 20, 2024
Title GS133_Placenta
Sample type SRA
 
Source name Placenta, LPS exposed, 24hrs post intra-amniotic injection
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
tissue: placenta
gestational day: Day 18
treatment: LPS O55:B5 1- micrograms
time: 24 hrs post-injection
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted from tissue homogenates with QIAGEN Rneasy kit per manufacturer's intructions
After polyA selection, paired-end RNA-sequencing with 150bp was done with a depth of 30M reads.Library was generated at BGI Genomics.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model DNBSEQ-G400
 
Description GS133_Placenta
Data processing Alignment with RN6.0 genome build with Kallisto
Gene summarization and feature counts with tximport
Differental expression with DESeq2
Assembly: Rn6.0
 
Submission date Jul 26, 2023
Last update date Mar 20, 2024
Contact name Augusto F Schmidt
E-mail(s) aschmidt@med.miami.edu
Phone 3052430156
Organization name University of Miami Miller School of Medicine
Street address 1611 NW 12th ave
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
 
Platform ID GPL30103
Series (1)
GSE239349 Transcriptional responses of the fetal lung after intra-amniotic LPS injection in rats [placenta]
Relations
BioSample SAMN36715874
SRA SRX21164290

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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