|
| Status |
Public on Jul 01, 2024 |
| Title |
3D primary GBM cells, 61_sham_2 |
| Sample type |
RNA |
| |
|
| Source name |
primary 3D GBM culture
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: sham
cell type: HuTuP61 GBM primary culture
|
| Treatment protocol |
After at least 20 days of growth GBM organoids were not exposed (sham) or exposed to two different milli-meter wave (30.5 GHz continuous wave [CW]) signals, including CW1 (incident power of 0.1 W [RMS] for 20 minutes) and CW2 (incident power of 0.2 W [RMS] for 10 minutes.
|
| Growth protocol |
Primary GBM cells were included in Matrigel and cultured for at least 20 days as 3D models in DMEM/F12 supplemented with 10% BIT9500, 20 ng/ml basic Fibroblast Growth Factor (bFGF) and 20 ng/ml Epidermal Growth Factor (EGF).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAzol and miRNeasy Micro Kit from Qiagen.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standardThermoFisher protocol starting from 100ng of Total RNA using the ThermoFisher WTA Kit
|
| |
|
| Hybridization protocol |
5.5ug of sscDNA were fragmentated and 5.2 ug were hybridized for 16 hr at 45°C on GeneChip Human Clariom S Array (GPL23159 Platform). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000.
|
| Description |
Gene expression data from untreated 3D primary GBM cells
|
| Data processing |
The data were normalized by TAC software using RMA and Repated Measure fuction for patients were applied
|
| |
|
| Submission date |
Jul 27, 2023 |
| Last update date |
Jul 01, 2024 |
| Contact name |
silvia bresolin |
| E-mail(s) |
silvia.bresolin@unipd.it
|
| Phone |
+390498215487
|
| Organization name |
università di padova
|
| Department |
department of women's and children's health
|
| Lab |
SSD ematologia-clinica sperimentale
|
| Street address |
via giustiniani 3
|
| City |
padova |
| State/province |
padova |
| ZIP/Postal code |
35128 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE239486 |
On the effects of 30 GHz sinusoidal wave exposure on glioblastoma organoids |
|
