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Sample GSM7666324 Query DataSets for GSM7666324
Status Public on Apr 18, 2024
Title 2_1(P2) treated A549
Sample type RNA
 
Source name miRNA from P2S peptide treated A549 cells
Organism Homo sapiens
Characteristics cell line: A549
cell type: Human Lung cancer cell line
treatment: P2S-peptide treated
Treatment protocol A549 cells were treated with blocking peptides-P2 and scramble-P2 (10μM daily) for 2day, then harvested and extracted total RNA for miRNA microarray 4.0 array.
Growth protocol A549 were propagated in DMEM medium according to standard protocols.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated miRNA were prepared using FlashTag Biotin HSR RNA Labeling Kit.
 
Hybridization protocol 21.5 μLof Biotin-labeled sample were hybridized for 16 hr at 48℃ on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G.
Description 2_1(P2S) [2_1.rma-dabg.chp Signal]
Data processing Affymetrix GeneChip Command Console software (version4.0, Affymetrix) was used to analyze array images to get raw data and then Affymetrix Expression Console 1.3.1. for normalization; Partek Genomics Suite software for statistical analysis
 
Submission date Jul 28, 2023
Last update date Apr 18, 2024
Contact name meng chieh lin
E-mail(s) kiwidopon@gmail.com
Organization name National Health Research Institutes
Street address 35 Keyan Road
City Miaoli
ZIP/Postal code 350
Country Taiwan
 
Platform ID GPL21572
Series (1)
GSE239499 miRNA differentially expressed in P2-peptide and P2S-peptide treated A549 cancer cells.

Data table header descriptions
ID_REF
VALUE rma-dabg.chp Signal

Data table
ID_REF VALUE
20500112 12.43
20500113 0.06
20500114 1.55
20500115 12.68
20500116 1.48
20500117 11.83
20500118 -0.07
20500119 11.1
20500120 2.96
20500121 12.23
20500122 2.81
20500123 8.79
20500124 0.89
20500125 0.08
20500126 6.85
20500127 0.9
20500128 11.43
20500129 0.44
20500130 10.85
20500131 4.12

Total number of rows: 6631

Table truncated, full table size 91 Kbytes.




Supplementary file Size Download File type/resource
GSM7666324_2_1_P2S_.CEL.gz 651.9 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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