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Status |
Public on Apr 18, 2024 |
Title |
3_2(P2) treated A549 |
Sample type |
RNA |
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Source name |
miRNA from P2 peptide treated A549 cells
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Organism |
Homo sapiens |
Characteristics |
cell line: A549 cell type: Human Lung cancer cell line treatment: P2-peptide treated
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Treatment protocol |
A549 cells were treated with blocking peptides-P2 and scramble-P2 (10μM daily) for 2day, then harvested and extracted total RNA for miRNA microarray 4.0 array.
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Growth protocol |
A549 were propagated in DMEM medium according to standard protocols.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated miRNA were prepared using FlashTag Biotin HSR RNA Labeling Kit.
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Hybridization protocol |
21.5 μLof Biotin-labeled sample were hybridized for 16 hr at 48℃ on miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 7G.
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Description |
3_2(P2S) [3_2.rma-dabg.chp Signal]
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Data processing |
Affymetrix GeneChip Command Console software (version4.0, Affymetrix) was used to analyze array images to get raw data and then Affymetrix Expression Console 1.3.1. for normalization; Partek Genomics Suite software for statistical analysis
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Submission date |
Jul 28, 2023 |
Last update date |
Apr 18, 2024 |
Contact name |
meng chieh lin |
E-mail(s) |
kiwidopon@gmail.com
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Organization name |
National Health Research Institutes
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Street address |
35 Keyan Road
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City |
Miaoli |
ZIP/Postal code |
350 |
Country |
Taiwan |
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Platform ID |
GPL21572 |
Series (1) |
GSE239499 |
miRNA differentially expressed in P2-peptide and P2S-peptide treated A549 cancer cells. |
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