|
| Status |
Public on May 15, 2024 |
| Title |
mouse lung_PBS_TJ41 rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
genotype: wild type
treatment 1: PBS
treatment 2: TJ4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to the instructions of the manufacturer of the RNeasy Mini Kit (Qiagen, Netherlands).
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
CLC Genomics Workbench v 21.0.5
RNA-sequencing (RNA-seq) reads were analyzed using CLC Genomics workbench software (Qiagen, Hilden, Germany) .
Read mapping was carried out using HISAT2 against mouse genome mm10.
Differential expression analysis was performed using edgeR Bioconductor package.
Assembly: mm10
Supplementary files format and content: a csv file include normalized values for each sample
|
| |
|
| Submission date |
Jul 28, 2023 |
| Last update date |
May 15, 2024 |
| Contact name |
Masaaki Yuki |
| E-mail(s) |
masaaki.yuki.702@gmail.com
|
| Phone |
09072744215
|
| Organization name |
The University of Tokyo
|
| Department |
Respiratory medicine
|
| Street address |
7-3-1 Hongo, Bunkyo-ku Tokyo
|
| City |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE239537 |
A Japanese herbal medicine, Hochuekkito (TJ-41), has anti-inflammatory effects on the COPD mouse model |
|
| Relations |
| BioSample |
SAMN36749420 |
| SRA |
SRX21185605 |
