|
| Status |
Public on Mar 19, 2024 |
| Title |
human T_ALL PDX from mouse 3 treated with control IgG2a |
| Sample type |
RNA |
| |
|
| Source name |
T_ALL tumor from human patient
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: CD45+ CD7+ tumor cell sorted from bone marrow
treatment: control antibody IgG2a
mouse id: JGL_ISO_91
|
| Treatment protocol |
T-ALL xenografted mice were treated for 6h with either 40µg control IgG2a, or OKT3 or Teplizumab. Mice were killed and leukemic cells were flow cytometry-sorted (hCD45+; hCD7+).
|
| Growth protocol |
NSG female mice were tail vein injected (1 million cells/mouse) with human T-ALL cells (hCD45+; hCD7+) and treated when tumor burden reached around 1% in blood.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single strand cDNA targets were prepared starting from 200 ng of total RNA (Rneasy kit QIAGEN) using the Ambion WT Expression Kit according to Affymetrix recommendations.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA were labeled with the Affymetrix GeneChip WT Terminal Labeling Kit according to Affymetrix recommendations.
|
| |
|
| Hybridization protocol |
3 μg of cDNAs were hybridized for 16 hours at 45C on GeneChip Human Gene 2.0 ST arrays (Affymetrix).
|
| Scan protocol |
The chips were stained in the GeneChip Fluidics Station 450 (Affymetrix) and scanned with the GeneChip Scanner 3000 7G (Affymetrix) at a resolution of 0.7 µm.
|
| Data processing |
Raw data were extracted from the scanned images using the Affymetrix GeneChip® Command Console (AGCC) version 4.1.2. CEL files were further processed with Affymetrix Expression Console software to calculate probe set signal intensities using Robust Multi-array Average (RMA) algorithms with default settings.
RMA expression estimated from expression Console software version 1.4.1. RMA algorithms with default settings.
|
| |
|
| Submission date |
Aug 07, 2023 |
| Last update date |
Mar 19, 2024 |
| Contact name |
Christine Tran Quang |
| E-mail(s) |
christine.tran-quang@curie.fr
|
| Organization name |
Institut Curie
|
| Street address |
Bat 110 centre universitaire
|
| City |
Orsay |
| ZIP/Postal code |
91405 |
| Country |
France |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE240261 |
Expression data from T-cell Acute Lymphoblastic Leukemia (T-ALL) patient-derived xenograft treated with anti-CD3 mAbs. |
|
