|
| Status |
Public on Nov 22, 2011 |
| Title |
E2B |
| Sample type |
RNA |
| |
|
| Source name |
aneuploid, breast adenocarcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
group: E2
cell line: MCF7
|
| Treatment protocol |
Where indicated, cells were pretreated with 50 nM Bortezomib for 15 minutes followed by 10 nM 17-β-estradiol for 24 hours.
|
| Growth protocol |
Cells were grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 48 hours prior to treatment with Bortezomib or estrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Nugen protocol from 300 ng total RNA using the Applause WT-Amp ST RNA amplification kit (Cat. N° 5500-24) and the Encore Biotin Module Kit (Cat. N° 4200-12). Wash and stain was performed by using the GeneChip Fluidic Station 450 from Affymetrix
|
| |
|
| Hybridization protocol |
Hybridizations were performed on the GeneChip Hybridization oven 645 (Affymetrix) at45º C for 17 hours.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000
|
| Data processing |
log2 transformation of signal intensity, quantile normalization, R/Bioconductor
|
| |
|
| Submission date |
Aug 02, 2011 |
| Last update date |
Nov 22, 2011 |
| Contact name |
Frank Kramer |
| E-mail(s) |
frank.kramer@med.uni-goettingen.de
|
| Organization name |
University Medical Center Göttingen
|
| Department |
Department of Medical Statistics
|
| Street address |
Humboldtallee 32
|
| City |
Goettingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14010 |
| Series (1) |
| GSE31118 |
Estrogen-dependent gene expression in human breast cancer cells relies upon proteosome-dependent monoubiquitination of histone H2B |
|
