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Status |
Public on Nov 22, 2011 |
Title |
siRNA_Ctrl1C |
Sample type |
RNA |
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|
Source name |
aneuploid, breast adenocarcinoma cell line
|
Organism |
Homo sapiens |
Characteristics |
group: siRNA_Ctrl1 cell line: MCF7
|
Treatment protocol |
Where indicated, cells were pretreated with 50 nM Bortezomib for 15 minutes followed by 10 nM 17-β-estradiol for 24 hours.
|
Growth protocol |
Cells were grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 48 hours prior to treatment with Bortezomib or estrogen.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
Label |
Biotin
|
Label protocol |
Biotinylated cDNA were prepared according to the Nugen protocol from 300 ng total RNA using the Applause WT-Amp ST RNA amplification kit (Cat. N° 5500-24) and the Encore Biotin Module Kit (Cat. N° 4200-12). Wash and stain was performed by using the GeneChip Fluidic Station 450 from Affymetrix
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|
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Hybridization protocol |
Hybridizations were performed on the GeneChip Hybridization oven 645 (Affymetrix) at45º C for 17 hours.
|
Scan protocol |
Affymetrix GeneChip Scanner 3000
|
Data processing |
log2 transformation of signal intensity, quantile normalization, R/Bioconductor
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Submission date |
Aug 02, 2011 |
Last update date |
Nov 22, 2011 |
Contact name |
Frank Kramer |
E-mail(s) |
frank.kramer@med.uni-goettingen.de
|
Organization name |
University Medical Center Göttingen
|
Department |
Department of Medical Statistics
|
Street address |
Humboldtallee 32
|
City |
Goettingen |
ZIP/Postal code |
37073 |
Country |
Germany |
|
|
Platform ID |
GPL14010 |
Series (1) |
GSE31118 |
Estrogen-dependent gene expression in human breast cancer cells relies upon proteosome-dependent monoubiquitination of histone H2B |
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