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Sample GSM770582 Query DataSets for GSM770582
Status Public on Nov 22, 2011
Title siRNA_Ctrl1C
Sample type RNA
Source name aneuploid, breast adenocarcinoma cell line
Organism Homo sapiens
Characteristics group: siRNA_Ctrl1
cell line: MCF7
Treatment protocol Where indicated, cells were pretreated with 50 nM Bortezomib for 15 minutes followed by 10 nM 17-β-estradiol for 24 hours.
Growth protocol Cells were grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 48 hours prior to treatment with Bortezomib or estrogen.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cDNA were prepared according to the Nugen protocol from 300 ng total RNA using the Applause WT-Amp ST RNA amplification kit (Cat. N° 5500-24) and the Encore Biotin Module Kit (Cat. N° 4200-12). Wash and stain was performed by using the GeneChip Fluidic Station 450 from Affymetrix
Hybridization protocol Hybridizations were performed on the GeneChip Hybridization oven 645 (Affymetrix) at45º C for 17 hours.
Scan protocol Affymetrix GeneChip Scanner 3000
Data processing log2 transformation of signal intensity, quantile normalization, R/Bioconductor
Submission date Aug 02, 2011
Last update date Nov 22, 2011
Contact name Frank Kramer
Organization name University Medical Center Göttingen
Department Department of Medical Statistics
Street address Humboldtallee 32
City Goettingen
ZIP/Postal code 37073
Country Germany
Platform ID GPL14010
Series (1)
GSE31118 Estrogen-dependent gene expression in human breast cancer cells relies upon proteosome-dependent monoubiquitination of histone H2B

Data table header descriptions
VALUE quantile normalized log2 signal intensity

Data table
100009676_at 6.490002204
10000_at 2.444412051
10001_at 9.331839188
10002_at 5.30182623
100033413_at 2.071123763
100033414_at 5.343957679
100033416_at 2.747089394
100033418_at 3.019471901
100033420_at 2.384913962
100033422_at 2.03979457
100033423_at 2.876250528
100033424_at 2.045014878
100033425_at 1.755564212
100033426_at 1.978434766
100033427_at 3.873990187
100033428_at 1.602175173
100033430_at 1.557200773
100033431_at 2.31459721
100033432_at 2.120846741
100033433_at 1.866505139

Total number of rows: 19628

Table truncated, full table size 399 Kbytes.

Supplementary file Size Download File type/resource
GSM770582_siRNA_Ctrl1C_TAL_Nr_12_HuGene-1_0-st-v1_.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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