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Sample GSM770586 Query DataSets for GSM770586
Status Public on Nov 22, 2011
Title siRNA_PSMB3A
Sample type RNA
Source name aneuploid, breast adenocarcinoma cell line
Organism Homo sapiens
Characteristics group: siRNA_PSMB3
cell line: MCF7
Treatment protocol Where indicated, cells were pretreated with 50 nM Bortezomib for 15 minutes followed by 10 nM 17-β-estradiol for 24 hours.
Growth protocol Cells were grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 48 hours prior to treatment with Bortezomib or estrogen.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cDNA were prepared according to the Nugen protocol from 300 ng total RNA using the Applause WT-Amp ST RNA amplification kit (Cat. N° 5500-24) and the Encore Biotin Module Kit (Cat. N° 4200-12). Wash and stain was performed by using the GeneChip Fluidic Station 450 from Affymetrix
Hybridization protocol Hybridizations were performed on the GeneChip Hybridization oven 645 (Affymetrix) at45º C for 17 hours.
Scan protocol Affymetrix GeneChip Scanner 3000
Data processing log2 transformation of signal intensity, quantile normalization, R/Bioconductor
Submission date Aug 02, 2011
Last update date Nov 22, 2011
Contact name Frank Kramer
Organization name University Medical Center Göttingen
Department Department of Medical Statistics
Street address Humboldtallee 32
City Goettingen
ZIP/Postal code 37073
Country Germany
Platform ID GPL14010
Series (1)
GSE31118 Estrogen-dependent gene expression in human breast cancer cells relies upon proteosome-dependent monoubiquitination of histone H2B

Data table header descriptions
VALUE quantile normalized log2 signal intensity

Data table
100009676_at 6.946829449
10000_at 2.540964812
10001_at 9.094842077
10002_at 4.995931736
100033413_at 2.21759588
100033414_at 4.590421257
100033416_at 2.813982325
100033418_at 2.520373107
100033420_at 2.093388586
100033422_at 2.05115544
100033423_at 2.354470408
100033424_at 2.247770774
100033425_at 1.98240283
100033426_at 2.136025446
100033427_at 3.809003418
100033428_at 1.953395632
100033430_at 2.075888342
100033431_at 2.51849277
100033432_at 2.063584631
100033433_at 1.76618205

Total number of rows: 19628

Table truncated, full table size 399 Kbytes.

Supplementary file Size Download File type/resource
GSM770586_siRNA_PSMB3A_TAL_Nr_16_HuGene-1_0-st-v1_.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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