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Sample GSM770587 Query DataSets for GSM770587
Status Public on Nov 22, 2011
Title siRNA_PSMB3B
Sample type RNA
 
Source name aneuploid, breast adenocarcinoma cell line
Organism Homo sapiens
Characteristics group: siRNA_PSMB3
cell line: MCF7
Treatment protocol Where indicated, cells were pretreated with 50 nM Bortezomib for 15 minutes followed by 10 nM 17-β-estradiol for 24 hours.
Growth protocol Cells were grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 48 hours prior to treatment with Bortezomib or estrogen.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cDNA were prepared according to the Nugen protocol from 300 ng total RNA using the Applause WT-Amp ST RNA amplification kit (Cat. N° 5500-24) and the Encore Biotin Module Kit (Cat. N° 4200-12). Wash and stain was performed by using the GeneChip Fluidic Station 450 from Affymetrix
 
Hybridization protocol Hybridizations were performed on the GeneChip Hybridization oven 645 (Affymetrix) at45º C for 17 hours.
Scan protocol Affymetrix GeneChip Scanner 3000
Data processing log2 transformation of signal intensity, quantile normalization, R/Bioconductor
 
Submission date Aug 02, 2011
Last update date Nov 22, 2011
Contact name Frank Kramer
E-mail(s) frank.kramer@med.uni-goettingen.de
Organization name University Medical Center Göttingen
Department Department of Medical Statistics
Street address Humboldtallee 32
City Goettingen
ZIP/Postal code 37073
Country Germany
 
Platform ID GPL14010
Series (1)
GSE31118 Estrogen-dependent gene expression in human breast cancer cells relies upon proteosome-dependent monoubiquitination of histone H2B

Data table header descriptions
ID_REF
VALUE quantile normalized log2 signal intensity

Data table
ID_REF VALUE
100009676_at 7.115336941
10000_at 2.775457106
10001_at 9.071355021
10002_at 5.321377247
100033413_at 2.258506414
100033414_at 4.662107054
100033416_at 2.747089394
100033418_at 2.477148233
100033420_at 2.306263309
100033422_at 2.236844259
100033423_at 3.071335115
100033424_at 2.491446657
100033425_at 1.851718381
100033426_at 2.247939972
100033427_at 4.003305757
100033428_at 1.959773368
100033430_at 1.807435539
100033431_at 2.51849277
100033432_at 2.420502425
100033433_at 1.853600199

Total number of rows: 19628

Table truncated, full table size 399 Kbytes.




Supplementary file Size Download File type/resource
GSM770587_siRNA_PSMB3B_TAL_Nr_17_HuGene-1_0-st-v1_.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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