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Sample GSM770589 Query DataSets for GSM770589
Status Public on Nov 22, 2011
Title siRNA_PSMB5A
Sample type RNA
Source name aneuploid, breast adenocarcinoma cell line
Organism Homo sapiens
Characteristics group: siRNA_PSMB5
cell line: MCF7
Treatment protocol Where indicated, cells were pretreated with 50 nM Bortezomib for 15 minutes followed by 10 nM 17-β-estradiol for 24 hours.
Growth protocol Cells were grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 48 hours prior to treatment with Bortezomib or estrogen.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cDNA were prepared according to the Nugen protocol from 300 ng total RNA using the Applause WT-Amp ST RNA amplification kit (Cat. N° 5500-24) and the Encore Biotin Module Kit (Cat. N° 4200-12). Wash and stain was performed by using the GeneChip Fluidic Station 450 from Affymetrix
Hybridization protocol Hybridizations were performed on the GeneChip Hybridization oven 645 (Affymetrix) at45º C for 17 hours.
Scan protocol Affymetrix GeneChip Scanner 3000
Data processing log2 transformation of signal intensity, quantile normalization, R/Bioconductor
Submission date Aug 02, 2011
Last update date Nov 22, 2011
Contact name Frank Kramer
Organization name University Medical Center Göttingen
Department Department of Medical Statistics
Street address Humboldtallee 32
City Goettingen
ZIP/Postal code 37073
Country Germany
Platform ID GPL14010
Series (1)
GSE31118 Estrogen-dependent gene expression in human breast cancer cells relies upon proteosome-dependent monoubiquitination of histone H2B

Data table header descriptions
VALUE quantile normalized log2 signal intensity

Data table
100009676_at 6.661366036
10000_at 2.278520317
10001_at 8.85937425
10002_at 5.411278092
100033413_at 1.908667148
100033414_at 4.190595316
100033416_at 2.639774884
100033418_at 2.674957993
100033420_at 2.357345195
100033422_at 2.106990879
100033423_at 2.98774916
100033424_at 2.368741973
100033425_at 1.790503795
100033426_at 1.868961424
100033427_at 4.172927868
100033428_at 1.700166319
100033430_at 1.671189996
100033431_at 2.50736821
100033432_at 2.025089251
100033433_at 1.756779492

Total number of rows: 19628

Table truncated, full table size 399 Kbytes.

Supplementary file Size Download File type/resource
GSM770589_siRNA_PSMB5A_TAL_Nr_19_HuGene-1_0-st-v1_.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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