|
| Status |
Public on May 17, 2024 |
| Title |
Adipo-2H |
| Sample type |
SRA |
| |
|
| Source name |
Bone
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone
cell line: MSC-8011L
cell type: Mesenchymal Stem Cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were dissociated from either hydrogels or tissue culture plasticware.
Single-cell rna-sequencing was performed using 10x Genomics Single Cell 3' Gene Expression protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The demultiplexing, barcoded processing and gene counting were made using the Cell Ranger software (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger)
Seurat was used for quality control analysis, data processing, and visualization.
Assembly: hg38
Supplementary files format and content: Data provided for single-cell RNA-Seq is the raw count matrix HDF5 file from Cell Ranger
|
| |
|
| Submission date |
Aug 17, 2023 |
| Last update date |
May 17, 2024 |
| Contact name |
Danh Truong |
| E-mail(s) |
dtruong4@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| ZIP/Postal code |
TX |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE241130 |
In Vitro Mesenchymal Stem Cell Differentiation Landscape |
|
| Relations |
| BioSample |
SAMN37041624 |
| SRA |
SRX21404641 |
