|
| Status |
Public on Mar 30, 2012 |
| Title |
TCF7_ChIPSeq |
| Sample type |
SRA |
| |
|
| Source name |
EML cells
|
| Organism |
Mus musculus |
| Characteristics |
sequence type: Illumina; single-end;35bp reads
antibody: TCF7 goat polyclonal antibody TCF1(H18)
vendor: Santa Cruz Biotechnology
catalog: SC8589
cell type: Lin-SCA+CD34+ cells
|
| Growth protocol |
EML cell culture was maintained in expansion medium (IMDB, 20% heat inactivated horse serum, 100 ng/ml SCF [PeproTech])
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cell lysates were clarified from sonicated nuclei and transcription factor-DNA complexes were isolated with antibody. DNA sequencing followed the instructions of the mRNA-Sequencing Sample Prep Kit (Illumina).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Chromatin IP against TCF7
|
| Data processing |
Alignment: Sequence reads were obtained and mapped to the mouse mm9 genomes using the Illumina Genome Analyzer Pipeline. All reads mapping with two or fewer mismatches were retained.
|
| |
|
| Submission date |
Aug 04, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiaqian Wu |
| E-mail(s) |
jiaqian2009.wu@gmail.com
|
| Organization name |
Stanford University
|
| Street address |
1501 California Avenue
|
| City |
PALO ALTO |
| ZIP/Postal code |
94306 |
| Country |
USA |
| |
|
| Platform ID |
GPL9185 |
| Series (1) |
| GSE31221 |
TCF7 is a key regulator of the switch of self-renewal and differentiation in a multipotential hematopoietic cell line |
|
| Relations |
| SRA |
SRX092472 |
| BioSample |
SAMN00710416 |
