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Sample GSM773994 Query DataSets for GSM773994
Status Public on Mar 30, 2012
Title TCF7_ChIPSeq
Sample type SRA
Source name EML cells
Organism Mus musculus
Characteristics sequence type: Illumina; single-end;35bp reads
antibody: TCF7 goat polyclonal antibody TCF1(H18)
vendor: Santa Cruz Biotechnology
catalog: SC8589
cell type: Lin-SCA+CD34+ cells
Growth protocol EML cell culture was maintained in expansion medium (IMDB, 20% heat inactivated horse serum, 100 ng/ml SCF [PeproTech])
Extracted molecule genomic DNA
Extraction protocol Cell lysates were clarified from sonicated nuclei and transcription factor-DNA complexes were isolated with antibody. DNA sequencing followed the instructions of the mRNA-Sequencing Sample Prep Kit (Illumina).
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
Description Chromatin IP against TCF7
Data processing Alignment: Sequence reads were obtained and mapped to the mouse mm9 genomes using the Illumina Genome Analyzer Pipeline. All reads mapping with two or fewer mismatches were retained.
Submission date Aug 04, 2011
Last update date May 15, 2019
Contact name Jiaqian Wu
Organization name Stanford University
Street address 1501 California Avenue
ZIP/Postal code 94306
Country USA
Platform ID GPL9185
Series (1)
GSE31221 TCF7 is a key regulator of the switch of self-renewal and differentiation in a multipotential hematopoietic cell line
SRA SRX092472
BioSample SAMN00710416

Supplementary file Size Download File type/resource
GSM773994_TCF7_ChIPSeq.bam 306.2 Mb (ftp)(http) BAM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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