|
| Status |
Public on Aug 29, 2023 |
| Title |
ac19 |
| Sample type |
SRA |
| |
|
| Source name |
Blood cell culture
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Blood cell culture
disease setting: rrLBCL
treatment: CART
|
| Extracted molecule |
other |
| Extraction protocol |
After completion of CAR T cell infusion, the infusion bag was washed with PBS to obtain residual cells for scRNA-seq and scTCR-seq.
Libraries were generated using a 10x Genomics Chromium Controller instrument and Chromium Single-Cell 3′ V5.1 reagent kits (10x Genomics) according to manufacturer's protocol.
RNA-Seq and TCR-seq
|
| |
|
| Library strategy |
OTHER |
| Library source |
other |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
Transcript level counting with cell ranger v6.0.0
TCR contig annotations with cell ranger v6.0.0
Assembly: hg38
Supplementary files format and content: gene expression matrix
Supplementary files format and content: TCR contig annotations
|
| |
|
| Submission date |
Aug 28, 2023 |
| Last update date |
Aug 29, 2023 |
| Contact name |
Michael Green |
| E-mail(s) |
mgreen5@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Department |
Lymphoma/Myeloma
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE241783 |
A single cell atlas of CD19 chimeric antigen receptor T cells |
|
