|
| Status |
Public on Aug 09, 2014 |
| Title |
A47 |
| Sample type |
genomic |
| |
|
| Source name |
gDNA from whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
sample id: A47
gender: M
total cholesterol (tc): 303
triglycerides (tg): 182
ldl: 141.00
percent stenosis: >50%
admin note: CAD-II
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from whole blood using the Gentra Puregene Blood Kit (Qiagen). DNA quality and quantity was assessed using a Nanodrop Spectrophotometer ND-1000.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
CEL files and CHP files (generated by Affymetrix GTC 4.0 using the birdseed V2 algorithm). CNV analysis was generated by PARTEK Genomic Suite 6.5 using genomic segmentation algorithm (CN5) by default parameters (CNCHP files).
|
| |
|
| Submission date |
Aug 09, 2011 |
| Last update date |
Aug 09, 2014 |
| Contact name |
Wei-Chung Shia |
| Organization name |
Changhua Christian Hospital
|
| Department |
Cancer Research Center
|
| Street address |
8F., No.235, Xuguang Rd.
|
| City |
Changhua City |
| ZIP/Postal code |
500 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE31276 |
Genetic Copy Number Variants in Myocardial Infarction Patients with Hyperlipidemia |
|
