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Sample GSM7776906 Query DataSets for GSM7776906
Status Public on Sep 13, 2023
Title rat#5-control_cortex_7d
Sample type RNA
Source name brain tissue
Organism Rattus norvegicus
Characteristics animal: rat#5
Sex: male
strain: Wistar
treatment: TBI
tissue: brain
brain region: cortex
activation agent: PBS treatment
time: 7 days
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Mini Kit followed by DNase I treatment.
Label SYBR Green
Label protocol cDNA was synthesized using RevertAid First Strand cDNA Synthesis Kit (TermoFisher) by corresponding standard protocol. The PCRs set with qPCRmix-HS SYBR master mixes (Evrogen, Russia) were carried out in a Bio-Rad cfx96 (BioRad, USA) in the following mode: primary denaturation 95 °C for 5 min; denaturation 95 °C 20 s, annealing 56–63 °C 20 s, elongation 72 °C 20 s (40 cycles).
Hybridization protocol n/a
Scan protocol n/a
Description control
Data processing For the normalization it uses the average of housekeeping genes: GAPDH, ACTB.
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)
Fold Change worksheet reports control/test (median all samples PBS treatment/ median all samples EV treatment) ratios at 7 and 14 days for brain regions (cortex, striatum, hippocampus).
Genes that did not show expression were excluded.
Submission date Sep 12, 2023
Last update date Sep 13, 2023
Contact name Diana Salikhova
Organization name RUDN University
Department Institute of molecular and cellular medecine
Lab Laboratory of cellular biotechnology
Street address Miklukho-Maklaya, 8
City Moscow
ZIP/Postal code 117198
Country Russia
Platform ID GPL33755
Series (1)
GSE242996 Real-time quantitative PCR analysis of rat's brain mRNAs

Data table header descriptions
VALUE normalized signal

Data table
A01 0.062292324
A02 0.058045713
B01 0.001428384
B02 0.003349292
B03 0.00192179
B04 0.000287943
E01 1.73834E-05
E02 0.021995212
F01 0.00284677
G01 0.000345267
G02 5.56471E-05
G03 0.005311494
G04 0.01991501
H01 0.002318
H02 0.000200608
H03 0.168404197
H04 0.072964238
H05 0.002393493
H06 0.012006837
H07 0.006896117

Total number of rows: 20

Table truncated, full table size <1 Kbytes.

Supplementary data files not provided

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