animal: rat#5 Sex: male strain: Wistar treatment: TBI tissue: brain brain region: cortex activation agent: PBS treatment time: 7 days
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted with RNeasy Mini Kit followed by DNase I treatment.
Label
SYBR Green
Label protocol
cDNA was synthesized using RevertAid First Strand cDNA Synthesis Kit (TermoFisher) by corresponding standard protocol. The PCRs set with qPCRmix-HS SYBR master mixes (Evrogen, Russia) were carried out in a Bio-Rad cfx96 (BioRad, USA) in the following mode: primary denaturation 95 °C for 5 min; denaturation 95 °C 20 s, annealing 56–63 °C 20 s, elongation 72 °C 20 s (40 cycles).
Hybridization protocol
n/a
Scan protocol
n/a
Description
control SAMPLE 1
Data processing
For the normalization it uses the average of housekeeping genes: GAPDH, ACTB. Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG) Fold Change worksheet reports control/test (median all samples PBS treatment/ median all samples EV treatment) ratios at 7 and 14 days for brain regions (cortex, striatum, hippocampus). Genes that did not show expression were excluded.
