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Sample GSM7776908 Query DataSets for GSM7776908
Status Public on Sep 13, 2023
Title rat#2-control_cortex_7d
Sample type RNA
 
Source name brain tissue
Organism Rattus norvegicus
Characteristics animal: rat#2
Sex: male
strain: Wistar
treatment: TBI
tissue: brain
brain region: cortex
activation agent: PBS treatment
time: 7 days
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Mini Kit followed by DNase I treatment.
Label SYBR Green
Label protocol cDNA was synthesized using RevertAid First Strand cDNA Synthesis Kit (TermoFisher) by corresponding standard protocol. The PCRs set with qPCRmix-HS SYBR master mixes (Evrogen, Russia) were carried out in a Bio-Rad cfx96 (BioRad, USA) in the following mode: primary denaturation 95 °C for 5 min; denaturation 95 °C 20 s, annealing 56–63 °C 20 s, elongation 72 °C 20 s (40 cycles).
 
Hybridization protocol n/a
Scan protocol n/a
Description control
SAMPLE 3
Data processing For the normalization it uses the average of housekeeping genes: GAPDH, ACTB.
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)
Fold Change worksheet reports control/test (median all samples PBS treatment/ median all samples EV treatment) ratios at 7 and 14 days for brain regions (cortex, striatum, hippocampus).
Genes that did not show expression were excluded.
 
Submission date Sep 12, 2023
Last update date Sep 13, 2023
Contact name Diana Salikhova
E-mail(s) diana_salikhova@bk.ru
Organization name RUDN University
Department Institute of molecular and cellular medecine
Lab Laboratory of cellular biotechnology
Street address Miklukho-Maklaya, 8
City Moscow
ZIP/Postal code 117198
Country Russia
 
Platform ID GPL33755
Series (1)
GSE242996 Real-time quantitative PCR analysis of rat's brain mRNAs

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
A01 0.041617477
A02 0.028529431
B01 0.001328043
B02 0.000101939
B03 0.001469968
B04 1.59436E-05
E01 2.03013E-06
E02 0.009843133
F01 0.00118668
G01 0.000947666
G02 7.70774E-05
G03 0.005975741
G04 0.237061508
H01 0.0016462
H02 0.001780708
H03 0.229516005
H04 0.268563162
H05 0.000696945
H06 0.003022588
H07 0.00283979

Total number of rows: 20

Table truncated, full table size <1 Kbytes.




Supplementary data files not provided

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