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Sample GSM7776925 Query DataSets for GSM7776925
Status Public on Sep 13, 2023
Title rat#12-EV_cortex_7d
Sample type RNA
Source name brain tissue
Organism Rattus norvegicus
Characteristics animal: rat#12
Sex: male
strain: Wistar
treatment: TBI
tissue: brain
brain region: cortex
activation agent: EV treatment
time: 7 days
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Mini Kit followed by DNase I treatment.
Label SYBR Green
Label protocol cDNA was synthesized using RevertAid First Strand cDNA Synthesis Kit (TermoFisher) by corresponding standard protocol. The PCRs set with qPCRmix-HS SYBR master mixes (Evrogen, Russia) were carried out in a Bio-Rad cfx96 (BioRad, USA) in the following mode: primary denaturation 95 °C for 5 min; denaturation 95 °C 20 s, annealing 56–63 °C 20 s, elongation 72 °C 20 s (40 cycles).
Hybridization protocol n/a
Scan protocol n/a
Description Test
Data processing For the normalization it uses the average of housekeeping genes: GAPDH, ACTB.
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)
Fold Change worksheet reports control/test (median all samples PBS treatment/ median all samples EV treatment) ratios at 7 and 14 days for brain regions (cortex, striatum, hippocampus).
Genes that did not show expression were excluded.
Submission date Sep 12, 2023
Last update date Sep 13, 2023
Contact name Diana Salikhova
Organization name RUDN University
Department Institute of molecular and cellular medecine
Lab Laboratory of cellular biotechnology
Street address Miklukho-Maklaya, 8
City Moscow
ZIP/Postal code 117198
Country Russia
Platform ID GPL33755
Series (1)
GSE242996 Real-time quantitative PCR analysis of rat's brain mRNAs

Data table header descriptions
VALUE normalized signal

Data table
A01 0.025796813
A02 0.005423112
B01 0.000531861
B02 0.000654798
B03 0.000465156
B04 8.93609E-05
E01 0.000238014
E02 0.032803722
F01 0.000185
G01 0.000813635
G02 2.1233E-05
G03 0.01316943
G04 0.123279373
H01 0.001843509
H02 0.00023967
H03 0.047147879
H04 0.024069278
H05 0.00060393
H06 0.003338317
H07 0.008974227

Total number of rows: 20

Table truncated, full table size <1 Kbytes.

Supplementary data files not provided

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